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Living Modified Organism (LMO)
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Bt okra
EN
OE-17A
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OrganizationMaharashtra Hybrid Seeds Company Limited (Mahyco)Private sector (business and industry), Head OfficeJalna - Aurangabad Road, Post Box no. 76, Dawalwadi, JalnaDawalwadi, Maharashtra
431203, IndiaPhone: +91 02482-262471,Fax: +91 02482-26002,Email: info@mahyco.com,Website: https://mahyco.com/,
The okra (Abelmoschus esculentus) was modified for resistance to Lepidoptera pests (e.g. spotted bollworm) through the expression of Bacillus thuringiensis Cry1Ac. The protein forms pores in the midgut lining of susceptible pests, leading to cell lysis and septicemia. Additionally, the okra contains an Escherichia coli neomycin phosphotransferase II cassette for kanamycin selection during transformation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-115629-1 Organism Abelmoschus esculentus - Okra, Lady's finger
pC2300cry1Ac10518ve
EN
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated
forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis - Bt, Bacillus, BACTUProtein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | (Bacteria)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoterPromoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminatorTerminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoterPromoter
The modified okra includes two gene cassettes: Bacillus thuringiensis cry1Ac and Escherichia coli neomycin phosphotransferase II (nptII).
The cry1Ac is under control of the Cauliflower mosaic virus (CaMV) 35S enhanced promoter and a poly(A) terminator. Due to the constitutive nature of the promoter, transcription is expected to occur at high levels.
Transcription of nptII is under control of the CaMV 35S promoter and 35S terminator. Again, transcription is expected to occur at high levels due to the nature of the viral promoter.
The cry1Ac is under control of the Cauliflower mosaic virus (CaMV) 35S enhanced promoter and a poly(A) terminator. Due to the constitutive nature of the promoter, transcription is expected to occur at high levels.
Transcription of nptII is under control of the CaMV 35S promoter and 35S terminator. Again, transcription is expected to occur at high levels due to the nature of the viral promoter.
- Food
- Transformation vector.pdf [ English ]
- Description from Bt okra from provisional patent.pdf [ English ]
- Sequences - provisional patent.pdf [ English ]
- Efficient genetic transformation of okra (Abelmoschus esculentus (L.) Moench) and generation of insect-resistant transgenic plants expressing the cry1Ac gene.PDF [ English ]
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