Loading...
You are viewing a DELETED record.
This record information is displayed for reference purpose only and should be not used.
This document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Male sterile Indian mustard
EN
bn 3.6
No
-
New Delhi,Organization:University of Delhi ()Academic or research institute, Centre for Genetic Manipulation of Crop Plants (CGMCP)
, IndiaPhone:Fax:Email:
The Indian mustard (Brassica juncea) was modified for male-sterility and herbicide tolerance. For male-sterility, the mustard expresses Bacillus amyloliquefaciens barnase, an RNase, in the tapetum cell layer of the pollen sac during anther development. Expression of the non-specific RNase, causes RNA to be degraded and leads to the failure of pollen development. Since female fertility is not expected to be impacted, this line can be used in the production of hybrid seeds because the plants cannot self-pollinate and thus require pollen from another parent.
For glufosinate tolerance, the mustard expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase, which inactivates the herbicide through acetylation.
The mustard variety RLM 198 was initially transformed with the barnase construct and then backcrossed into variety Varuna to achieve the parental line Varuna bn 3.6 for hybrid seed production. Six backcrossings were performed to try to remove integration of vector backbone sequence.
EN
For glufosinate tolerance, the mustard expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase, which inactivates the herbicide through acetylation.
The mustard variety RLM 198 was initially transformed with the barnase construct and then backcrossed into variety Varuna to achieve the parental line Varuna bn 3.6 for hybrid seed production. Six backcrossings were performed to try to remove integration of vector backbone sequence.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-115905-1 Organism Brassica juncea (Indian mustard, Brown mustard, Chinese mustard, Leaf mustard, Vegetable mustard, Mustard greens, BRAJU)Crops
RLM 198 was developed through mutation and recombination breeding by Punjab Agricultural University. Varuna (T-59) is extensively cultivated in northern India.
EN
pPZP200
EN
- Agrobacterium-mediated DNA transfer
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
3.000 kb
|
|
2.000 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-103886-2 5' Untranslated Leader of AMV RNA4 | Alfalfa mosaic virus (Alfalfa mosaic virus, AMV)Leader
-
BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
-
BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
-
BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
-
BCH-GENE-SCBD-115906-1 Topoisomerase | Pisum sativum (Garden pea, PEA)Protein coding sequence
-
BCH-GENE-SCBD-101407-6 pTA29 pollen specific promoter | Nicotiana tabacum (Tobacco, TOBAC )Promoter
-
BCH-GENE-SCBD-14973-6 Barnase | Bacillus amyloliquefaciens (BACAM)Protein coding sequence | Changes in physiology and/or production (Reproduction, Male sterility)
-
BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
-
BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
The modified brown mustard contains two gene cassettes: Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar) and Bacillus amyloliquefaciens barnase.
The bar coding sequence is under control of a Cauliflower mosaic virus (CaMV) 35S promoter with an Alflafa mosaic virus (AMV) leader and an Rhizobium radiobacter octopine synthase gene terminator. The AMV leader sequence enhances expression of bar.
Barnase is under control of a Nicotiana tabacum TA29 promoter and a CaMV 35S terminator. The TA29 promoter is active only in the tapetum cell layer of the pollen sac during anther development (male-specific expression).
A spacer fragment can be found between the two gene cassettes to prevent 'leaky' expression of barnase from the CaMV promoter. It is comprised of Pisum sativum topoisomerase (3kb) and Arabidopsis thaliana acetohydroxy acid synthase (2 kb) fragments. Each fragment contains truncations on the 3' and 5' ends. No open reading frames were created during the fusion of the two fragments and thus are not expected to encode a functional product. Note: The arrangement of the insert is unclear.
Note:
- Southern blot and segregation analyses indicated that the genome contains a single insertion.
- Sequencing analysis indicated that the T-DNA integrated was identical to the sequences in the vector.
- The T-DNA was found to be integrated in A9 Linkage Group on the 'A' genome between Bra32488 and Bra32489 genes.
EN
The bar coding sequence is under control of a Cauliflower mosaic virus (CaMV) 35S promoter with an Alflafa mosaic virus (AMV) leader and an Rhizobium radiobacter octopine synthase gene terminator. The AMV leader sequence enhances expression of bar.
Barnase is under control of a Nicotiana tabacum TA29 promoter and a CaMV 35S terminator. The TA29 promoter is active only in the tapetum cell layer of the pollen sac during anther development (male-specific expression).
A spacer fragment can be found between the two gene cassettes to prevent 'leaky' expression of barnase from the CaMV promoter. It is comprised of Pisum sativum topoisomerase (3kb) and Arabidopsis thaliana acetohydroxy acid synthase (2 kb) fragments. Each fragment contains truncations on the 3' and 5' ends. No open reading frames were created during the fusion of the two fragments and thus are not expected to encode a functional product. Note: The arrangement of the insert is unclear.
Note:
- Southern blot and segregation analyses indicated that the genome contains a single insertion.
- Sequencing analysis indicated that the T-DNA integrated was identical to the sequences in the vector.
- The T-DNA was found to be integrated in A9 Linkage Group on the 'A' genome between Bra32488 and Bra32489 genes.
EN
- Other (Hybrid seed production)
EN
EN
- Safety-assessment-report-on-GE-Mustard_0.pdf [ English ]
- GenBank - Binary cloning vector pPZP200 for plant transformation, complete sequence [ English ]
- SnapGene - pPZP200 [ English ]
| Record type | Field | Record(s) | |||||
|---|---|---|---|---|---|---|---|
|
|||||||
