Drought tolerant sugarcane | BCH-LMO-SCBD-116035 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 02 Feb 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Drought tolerant sugarcane
EN
NXI-4T
No
The sugarcane (Saccharum officinarum) was modified for abiotic (drought, salt) stress tolerance through the expression of Rhizobium meliloti choline dehydrogenase, which leads to increased glycine betaine biosynthesis. Glycine betaine maintains a cell's water potential by osmotic adjustment. The expression of choline dehydrogenase may also increase sugar content and promote early maturing.

The sugarcane also contains Escherichia coli neomycin phosphotransferase II and hygromycin B phosphotransferase for kanamycin and hygromycin resistance, respectively. 
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Sugarcane cultivar BL579
EN
  • Drought tolerant sugarcane
    | Changes in physiology and/or production (Ripening, Yield), Resistance to antibiotics (Hygromycin, Kanamycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought, Salinity)
  • Drought-tolerant sugarcane
    | Changes in physiology and/or production (Ripening, Yield), Resistance to antibiotics (Hygromycin, Kanamycin, Neomycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought, Salinity)
Characteristics of the modification process
pMLH2113
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-116036-1 Glutamate dehydrogenase mitochondrial transit peptide | Solanum lycopersicum (Tomato, SOLLC)
    Transit signal
  • BCH-GENE-SCBD-116039-1 Choline dehydrogenase | Sinorhizobium meliloti (RHIML)
    Protein coding sequence | Changes in physiology and/or production (Ripening, Yield),Tolerance to abiotic stress (Drought, Salinity)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)
    Leader
The modified sugarcane contains three gene cassettes: Rhizobium meliloti choline dehydrogenase (betA); Escherichia coli neomycin phosphotransferase (nptII) and E. coli hygromycin B phosphotransferae (hph).

The betA sequence is under control of a Cauiflower mosaic virus (CaMV) 35S promoter and Agrobacterium tumefaciens nopaline synthase (nos) terminator. At the 5' end of the betA coding sequence is a Tobacco mosaic virus omega 5' leader to enhance translation and a Solanum lycopersicum glutamate dehydrogenase mitochondrial transit peptide, which directs the translated protein to the mitochondria.

The hph coding sequence is under transcriptional control of a CaMV 35S promoter and terminator.

The nptII coding sequence is under transcriptional control of a nos promoter and terminator.

Important notes:
- The CaMV promoter associated with the betA coding sequence may have two tandem repeats of -419 to -90.
EN
LMO characteristics
EN
  • Biofuel
  • Food
Detection method(s)
EN
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