MON-8746Ø-4 × DAS-Ø15Ø7-1 - Drought-tolerant, herbicide-tolerant, insect-resistant maize | BCH-LMO-SCBD-116322 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 07 Oct 2021
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Drought-tolerant, herbicide-tolerant, insect-resistant maize
EN
MON87460 × TC1507
MON-8746Ø-4 × DAS-Ø15Ø7-1
The maize (Zea mays) was produced through cross breeding of modified parental maize lines for drought tolerance, herbicide tolerance and insect resistance. For abiotic tolerance, the maize expresses Bacillus subtillus cold shock protein to enhance natural abiotic (drought) stress responses. For herbicide tolerance, the maize expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which inactivates glufosinate through acetyalation. For Lepidoptera tolerance, the maize expresses Bacillus thuringiensis Cry1F.  Additionally, the maize contains an Escherichia coli neomycin phosphotransferase II cassette for kanamycin selection, which was used during transformation of a parental line.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
    Crops
  • BCH-LMO-SCBD-103066-6 Living Modified Organism MON-8746Ø-4 - Droughtgard™ Maize
    Resistance to antibiotics - Kanamycin Tolerance to abiotic stress - Cold / Heat, Drought
  • BCH-LMO-SCBD-14841-13 Living Modified Organism DAS-Ø15Ø7-1 - Herculex™ I maize
    Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Resistance to herbicides (Glufosinate)
EN
Characteristics of the modification process
PV-ZMAP595; PHI8999A
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from MON87460 vector PV-ZMAP595
The T-DNA insert contains the following gene cassettes: Bacillus subtillus cold shock protein (cspB) and Escherichia coli neomycin phosphotransferase II (nptII). 

Transcription of cspB is under control of the Oryza sativa actin 1 promoter and Agrobacterium tumefaciens transcript 7 gene 3' untranslated region. The transcript initially contains an O. sativa actin 1 intron for enhanced gene expression of cspB. The sequence is removed (spliced) prior to protein translation. Constitutive expression of cspB is expected due to the actin promoter.

Transcription of nptII is under control of the Cauliflower mosaic virus (CaMV) 35S promoter and A. tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the CaMV promoter.

Note:
- The coding sequence of cspB has been codon optimized for optimal expression within plant cells.
- Southern blot analysis indicated that no vector backbone sequences were inserted into the parental genome
- Southern blot analysis indicated that the parental genome contains a single insertion
- Sequencing analyses confirm the Southern blot analyses.
- A 22 base pair deletion of genomic DNA at the insert-to-plant DNA junction occurred.
- loxP sites can be found in the parental genome and could potentially allow for the excision of the nptII cassette by CRE recombinase.

DNA insert from TC1507 vector PHI8999A
DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for Bacillus thuringiensis cry1F and Streptomyces viridochromogenes pat.

Transcription of cry1F is directed by the promoter and first exon and intron of the maize (Zea mays) ubiquitin gene and terminates at the Agrobacterium tumefaciens ORF25 terminator.

Transcription of the pat gene commences from the Cauliflower mosaic virus (CaMV) 35S promoter and ends at the CaMV 35S terminator.

Note:
- The coding sequence of both genes has been optimized to achieve a high level of expression in maize.
- The sequences of the complete cry1F and pat are identical to those in the original plasmid.
- The CRY1F protein includes the F604K (phenylalanine to lysine at position 604) amino acid substitution, which was introduced to create a specific restriction site for cloning purposes.

For more information, kindly refer to the parental LMO records.
EN
LMO characteristics
EN
  • Food
  • Feed
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1