SYN-EV176-9 - NaturGard KnockOut™ maize | BCH-LMO-SCBD-14751 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 14 Aug 2012
Living Modified Organism identity
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NaturGard KnockOut™ maize
Bt176 (176)
This LMO contains two copies of a truncated synthetic version of the full length cry1Ab gene from Bacillus thuringiensis subsp. kurstaki. The synthetic truncated cry1Ab gene encodes a protein that corresponds to the first 648 amino acids of the N-terminal of the 1155 amino acid full length native Cry1Ab protein and includes the portion of the native protein that is necessary for insect control.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Proprietary Ciba Seeds inbred maize line CG00526
Characteristics of the modification process
pCIB3064 and pCIB4431
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-14975-5 Beta-lactamase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Ampicillin)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-101404-3 Phosphoenolpyruvate Carboxylase gene promoter | Zea mays (Maize, Corn, MAIZE)
  • BCH-GENE-SCBD-101405-2 Calcium-dependent protein kinase promoter | Zea mays (Maize, Corn, MAIZE)
  • BCH-GENE-SCBD-101406-4 Phosphoenolpyruvate carboxylase, intron 9 | Zea mays (Maize, Corn, MAIZE)
Additional information concerning the cry1Ab gene inserts in this LMO:
The expression of the two copies of the cry1Ab genes are under the control either of a pollen-specific promoter the from a calcium-dependent protein kinase or green tissue-specific promoter phosphoenolpyruvate carboxylase. Both promoters were isolated from maize. The termination sequences for both of genes was from cauliflower mosaic virus (CaMV), a known plant pest.

Additional information concerning the bar gene insert in this LMO:
This LMO contains one copy of the bar gene from Streptomyces hygroscopicus which encodes for phosphinotricin acetyltransferase (PAT) that confers resistance to glufosinate herbicide. The bar gene is under the regulation of the 35S promoter and the 35S terminator from the cauliflower mosaic virus (CaMV).

Additional information concerning the bla gene insert in this LMO:
The bla gene from Escherichia coli is not expressed in plant cells, but was employed as a selectable trait for screening bacterial colonies for the presence of the plasmid vector.

Additional information on the inserted genetic material:

Two plasmids, pCIB3064 and pCIB4431 were used as vectors for the transformation of Bt176 maize. Both are derivatives of the plasmid PUC18, which has a molecular weight of 2.7 kb and contains sequences such as prokaryotic gene bla and gene lacZ. 

Plasmid pCIB3064 contains one copy of the bar gene which is under the regulation of the 35S promoter and the 35S terminator from the cauliflower mosaic virus (CaMV).  The plasmid pCIB4431 contains two copies of a synthetic truncated cry1Ab gene; the first copy which is under the regulation of a promoter derived from maize phosphoenolpyruvate carboxylase gene and the CaMV 35S terminator; and the second copy which is under the regulation of a promoter derived from maize calcium-dependent protein kinase gene (“pollen promoter’) and the CaMV 35S terminator.

There are uncertainties regarding the copy number of the inserts in Bt176. Evidence suggests that 2-5 copies of the inserts may be present (see document below on the molecular characterization of Bt176).
LMO characteristics
  • Food
  • Feed
  • Biofuel
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 21
Country's Decision or any other Communication Living modified organism(s) 20
Risk Assessment generated by a regulatory process Living modified organism(s) 17