ACS-BNØØ3-6 - InVigor™ canola | BCH-LMO-SCBD-14755 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 30 May 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
InVigor™ canola
EN
RF3
ACS-BNØØ3-6
Canola fertility restoration system displaying glufosinate herbicide tolerance. Contains the barstar gene from Bacillus amyloliquefaciens, and the bar gene encoding phosphinothricin N-acetyltransferase (PAT) from Streptomyces hygroscopicus to confer tolerance to the herbicide phosphinothricin (Glufosinate ammonium).
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pTHW118
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
On the N-terminal  two codons of the wild type bar coding region have been substituted for the codons ATG and GAC.

Southern blot and PCR analysis indicated that the LMO contains one complete copy of the transformation cassette arranged in an inverted repeat orientation to a second partial copy (comprising of a portion of the TA29 promoter, the barstar coding sequence, the nos terminator sequence and a non-initiation codon of the bar gene)  of the transformation cassette adjacent to the left T-DNA boundary.
EN
LMO characteristics
EN
  • Food
  • Feed
Additional Information
The transgenic line RF3 was produced by genetically engineering plants to restore fertility in the hybrid line. Transgenic RF3 plants contain the barstar gene, isolated from Bacillus amyloliquefaciens. The barstar gene codes for a ribonuclease inhibitor (barstar enzyme) expressed only in the tapetum cells of the pollen sac during anther development. The ribonuclease inhibitor (barstar enzyme) specifically inhibits barnase RNAse expressed by male sterile lines. Together, the RNAse and the ribonuclease inhibitor form a very stable one-to-one complex, in which the RNAse is inactivated. As a result, when pollen from the restorer line RF3 is crossed to a male sterile line, the resultant progeny express the RNAse inhibitor in the tapetum cells of the anthers, allowing hybrid plants to develop normal anthers and restoring fertility.

RF3 was also engineered to express tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®). Glufosinate chemically resembles the amino acid glutamate and acts to inhibit an enzyme, called glutamine synthetase, which is involved in the synthesis of glutamine. Essentially, glufosinate acts enough like glutamate, the molecule used by glutamine synthetase to make glutamine, that it blocks the enzyme's usual activity. Glutamine synthetase is also involved in ammonia detoxification. The action of glufosinate results in reduced glutamine levels and a corresponding increase in concentrations of ammonia in plant tissues, leading to cell membrane disruption and cessation of photosynthesis resulting in plant withering and death. 

Glufosinate tolerance in this line was the result of introducing a gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces hygroscopicus. The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound.
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 14
Country's Decision or any other Communication Living modified organism(s) 18
Living Modified Organism Recipient Organism” or “Parental Organisms 9
Living Modified Organism Related LMO(s) 1
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 14
Risk Assessment generated by an independent or non-regulatory process Living modified organism(s) 1