ACS-BNØØ7-1 - Liberty-Link™ Innovator Canola | BCH-LMO-SCBD-14761 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
Decisions on the LMO Risk Assessments  
last updated: 05 Jun 2017
Living Modified Organism identity
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Liberty-Link™ Innovator Canola
Topas 19/2 (HCN92)
Glyphosate herbicide tolerant canola produced by inserting the phosphinothricin acetyltransferase (pat) gene conferring tolerance to Phosphinothricin (Glufosinate ammonium) herbicide and neomycin phosphotransferase II (npt II) conferring resistance to the herbicide kanamycin.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Southern blot analysis indicated that 2 copies of the T-DNA cassette was transformed into the host genome at a single insertion site. The 2 cassettes were arranged in an inverted RB/RB arrangement. Furthermore one of the two nptII genes, one was only partially integrated.
LMO characteristics
  • Food
  • Feed
Additional Information
The canola line HCN92 was genetically engineered to express tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®). Glufosinate chemically resembles the amino acid glutamate and acts to inhibit an enzyme, called glutamine synthetase, which is involved in the synthesis of glutamine. Essentially, glufosinate acts enough like glutamate, the molecule used by glutamine synthetase to make glutamine, that it blocks the enzyme's usual activity. Glutamine synthetase is also involved in ammonia detoxification. The action of glufosinate results in reduced glutamine levels and a corresponding increase in concentrations of ammonia in plant tissues, leading to cell membrane disruption and cessation of photosynthesis resulting in plant withering and death.

Glufosinate tolerance in HCN92 is the result of introducing a gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces viridochromogenes, the same organism from which glufosinate was originally isolated. The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound.
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 10
Country's Decision or any other Communication Living modified organism(s) 13
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 5
Living Modified Organism Related LMO(s) 1