PH-MON8Ø9-2 - Insect-resistant maize MON809 | BCH-LMO-SCBD-14779 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 24 May 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant maize MON809
EN
MON809
Yes
PH-MON8Ø9-2
Maize resistant to European corn borer (Ostrinia nubilalis) through introduction of a synthetic cry1Ab gene. Also tolerant to the glyphosate herbicide via introduction of the bacterial version of the epsps gene encoding a plant enzyme, 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) and glyphosate oxidoreductase (gox).
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • MON-ØØ81Ø-6 - YieldGard™ maize
    | Monsanto | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis))
  • Insect Resistant Maize MON801
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis))
Characteristics of the modification process
PV-ZMBK07 and PV-ZMGT10
EN
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-14998-4 Glyphosate oxidoreductase gene | Ochrobactrum anthropi (OCHAN)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)
    Intron
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-101902-4 rbcS Transit Peptide | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-101411-3 pUC origin of replication
    Plasmid Vector
The PV-ZMBK07 plasmid contained the coding sequence of a synthetic version of the cry1Ab gene from Bacillus thuringiensis, which was modified to enhance the expression of the Cry1Ab protein in plants, however the resulting amino acid sequence is identical to the native protein.

PV-ZMGT10 plasmid contained the genes cp4 epsps and gox as well as the antibiotic resistance marker gene nptII.

Southern blot analysis indicated that MON809 contains one full and one partial copy of the Cry1Ab coding sequence, two full copies of the EPSPS coding sequence and one partial copy of the gox gene sequence. Furthermore analysis indicated that portions of the phosphate backbone containing nptII and ori-pUC19 were also integrated into the host genome.
EN
LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
EN
Additional Information
The transgenic maize line MON809 was genetically engineered to resist ECB by producing its own insecticide. This line was developed by introducing the cry1Ab gene. Cry proteins, of which Cry1Ab is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. Cry1Ab is lethal only when eaten by the larvae of lepidopteran insects (moths and butterflies), and its specificity of action is directly attributable to the presence of specific binding sites in the target insects.
EN
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