Insect-resistant, herbicide-tolerant maize | BCH-LMO-SCBD-259066 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 02 Feb 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant, herbicide-tolerant maize
EN
SK12-5
No
The maize (Zea mays) was modified for 'double resistance' (insect-resistance, herbicide-tolerance). For Lepidoptera resistance, the maize expresses a fusion Bacillus thuringiensis crystal protein consisting of Cry1Ab and Cry2Aj (Cry1Ab-Cry2Aj). The protein is expected to act similarly to other crystal proteins and have a pore-forming mode of action. For tolerance to glyphosate, the maize expresses Deinococcus radiodurans 5-enolpyruvylshikimate-3-phosphate synthase gene G10, which does not bind glyphosate with high affinity and therefore allows for the continued functioning of the shikimate pathway (aromatic amino acid biosynthesis). 
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Maize inbred line Zheng58
EN
Characteristics of the modification process
pCAMBIA1300
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The modified maize contains two gene cassettes: a synthetic fusion Bacillus thuringiensis cry1ab/cry2aj and Deinococcus radiodurans 5-enolpyruvylshikimate-3-phosphate synthase gene G10 (G10-epsps).

The cry1ab/cry2aj coding sequence is under of a maize polyubiquitin promoter and a maize PEP carboxylase terminator. High levels of expression of cry1ab/cry2aj is expected in all plant tissues due to the constitutive nature of the promoter.

The G10-epsps coding sequence is under control of a fusion Cauliflower mosaic virus 35S-maize polyubiquitin promoter and a Cauliflower mosaic virus 35S terminator. An acetohydroxy acid synthase transit peptide is also included to target the G10-EPSPS protein to the chloroplast. High levels of expression are expected in all plant tissues due to the constitutive nature of the promoter.

Note:
  • The promoter of the epsps g10 cassette consists of a fusion of the CaMV 35S promoter and the maize ubiquitin promoter. The total size of the fused promoter is 2731 basepairs. It is was unclear what the size of the individual CaMV 35S and the ubiquitin portions were at the time of publication.
  • The transformation vector was a modified pCAMBIA1300 vector, where the hygromycin resistance cassette was replaced with the g10-epsps cassette.
  • Nanopore sequencing indicated that the T-DNA insert was putatively integrated into chromosome 9 at positions from 82,329,568 to 82,379,296 basepairs (Bin9.03 of chromosome 9 between the SSR markers umc2337 and umc1743 (26,822,048–100,724,531 bp)).
  • Crossing and nanopore sequencing analyses indicated that a single T-DNA is present in the SK12-5 genome.
EN
LMO characteristics
EN
  • Feed
  • Food
Detection method(s)
EN

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