CTC-95Ø19-5 - Insect-resistant sugarcane | BCH-LMO-SCBD-259285 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 24 Feb 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant sugarcane
EN
CTC95019-5
Yes
CTC-95Ø19-5
The event CTC95019-5 was obtained through the genetic transformation of the sugarcane (Saccharum officinarum L.) cultivar CTC9005HP for resistance to insects. To achieve Lepidoptera resistance, the sugarcane expresses Bacillus thuringiensis Cry1Ac, which forms pores in the midgut of larvae and leads to death. In addition, the modified sugarcane expresses Escherichia coli neomycin phosphotransferase II, which acts as a marker for kanamycin selection during transformation. 
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Sugarcane variety CTC9005HP
EN
  • CTC-932Ø9-4 - Insect resistant sugarcane
    | CTC - Centro de Tecnologia Canavieira(CTC) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • CTC-91Ø87-6 - Insect resistant sugarcane
    | CTC - Centro de Tecnologia Canavieira(CTC) | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Resistance to herbicides (Glufosinate), Selectable marker genes and reporter genes
Characteristics of the modification process
pCTC523
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
The sugarcane event was obtained through Agrobacterium tumefaciens-mediated transformation to transfer a DNA fragment (T-DNA) containing the expression cassettes for Bacillus thuringiensis cry1Ac and Escherichia coli nptII genes. The cry1Ac coding sequence is under control of the Zea mays ubiquitin promoter and Agrobacterium tumefaciens nopaline synthase terminator. The nptII coding sequence is under control of the Zea mays ubiquitin promoter and Agrobacterium tumefaciens nopaline synthase terminator.

Note:
  • The vector used to create CTC95019-5 is the same as CTC93204-9.
  • The orientation (direction) of the genetic constructs and number of T-DNA insertions is not available at this time.
EN
LMO characteristics
EN
  • Biofuel
  • Feed
  • Food
Detection method(s)
EN
Additional Information
EN
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