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Living Modified Organism
(LMO)
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Red fluorescent zebrafish
EN
TK-2-Red
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- Organization: Taikong Corporation (AZOO) | BCH-CON-SCBD-260323-1 This document has been updated. This is not the latest published version. Click here to view the latest version of the record.Organization:Taikong Corporation (AZOO)Private sector (business and industry)10F, No. 33, Lane 3, Sec. 1, ZhongZheng E. Rd. 251New Taipei City,
115, ChinaPhone: +886.2.26200806,Fax:Email: azoo.aqua88@gmail.com,
The zebrafish (Danio rerio) was modified for red fluorescent coloration through the expression of Discosoma sp. red fluorescent protein (DsRed2). The fluorescent zebrafish has a few different names depending on the variety of zebrafish used in the transformation and subsequent breeding; examples include "Red Fluorescent Fairy", "Purple Fluorescent Fairy", "Red Leopard Fluorescent Fairy", "Purple Leopard Fluorescent Fairy", "Platinum Red Leopard Fluorescent Fairy", "Red Gold Fluorescent Fairy", "Purple Gold Fluorescent Fairy" and "Red Ribbon Fluorescent Fairy".
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-45401-5 Organism Danio rerio (Zebra fish, leopard danio, zebra danio)Fish
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pZβ-DsRed2-1-ITR
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- Microinjection
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-260317-1 Inverted terminal repeats | Adeno-associated dependoparvovirus A (Adeno-associated virus 2)Inverted terminal repeats
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BCH-GENE-SCBD-259257-1 β actin promoter | Danio rerio (Zebra fish, leopard danio, zebra danio)Promoter
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BCH-GENE-SCBD-101476-6 DsRed2 Fluorescent Protein | Discosoma sp. (Coral anemones, Sea anemones)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-114748-3 SV40 poly-adenylation signal | Macaca mulatta polyomavirus 1 (SV40, Simian vacuolating virus 40, simian virus 40, Rhesus macaque polyomavirus)Terminator
The modified zebrafish is expresses Discosoma sp. red fluorescent protein (DsRed2). The coding sequence is under control of the zebrafish beta-actin promoter and Macaca mulatta polyomavirus 1 (SV40) terminator. The promoter is expected to promote high levels of transcription in all body tissues. The Adeno-associated virus inverted terminal repeats found at either side of the cassette were used for integrating the transgene sequences into the zebrafish genome.
Note: The donor species of Adeno-associated virus inverted terminal repeats was not specified in the patent document. Thus, Adeno-associated virus 2 was chosen as a placeholder due to its use in recombinant vectors until further information becomes available.
Note: The donor species of Adeno-associated virus inverted terminal repeats was not specified in the patent document. Thus, Adeno-associated virus 2 was chosen as a placeholder due to its use in recombinant vectors until further information becomes available.
EN
- Ornamental
EN
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