Herbicide-tolerant eucalpytus | BCH-LMO-SCBD-260712 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 17 Jun 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant eucalpytus
The eucalyptus (Eucalyptus globulus) was modified for herbicide tolerance through the expression of Rhizobium radiobacter (Agrobacterium tumefaciens) 5-enolpyruvylshikimate-3-phosphate synthase (epsps). The bacterial enzyme does not bind the herbicide glyphosate with high affinity (unlike the endogenous EPSPS protein) and thus allows for the continued biosynthesis of aromatic amino acids through the shikimate pathway during glyphosate applications. In addition to the epsps cassette, the eucalyptus also expresses Escherichia coli neomycin phosphotransferase II, which was used for kanamycin selection during transformation. 
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-102151-5 Organism Eucalyptus globulus (Eucalyptus, Tasmanian Blue Gum, Southern Blue Gum, Blue gum)
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The eucalyptus contains two gene cassettes: Rhizobium radiobacter 5-enolpyruvylshikimate-3-phosphate synthase (epsps) and Escherichia coli neomycin phosphotransferase (nptII).

The epsps coding sequence is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and R. radiobacter nopaline synthase terminator.

The nptII coding sequence is under control of a CaMV 35 promoter and terminator.

Due to the constitutive nature of the CaMV promoter, expression of EPSPS and NPTII are expected to occur at high levels.
LMO characteristics
  • Timber
Detection method(s)
Additional Information
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