Loading...
You are viewing a DELETED record.
This record information is displayed for reference purpose only and should be not used.
This document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant, insect-resistant, amylase-producing maize
EN
3272 × Bt11 × MIR162 × MIR604 × TC1506 × 5307 × GA21
Yes
SYN-E3272-5 × SYN-BTØ11-1 × SYN-IR162-4 × SYN-IR6Ø4-5 × DAS-Ø15Ø7-1 × SYN-Ø53Ø7-1 × MON-ØØØ21-9
-
Organization:Syngenta ()Phone:Fax:Email:Website: http://www.syngentaseeds.com,
-
Organization:Dow AgroSciences ()Phone:Fax:Email:
The maize (Zea mays) was produced by crossing modified parental lines for tolerance to herbicides, resistance to insect pests and improved use in biofuel. For tolerance to Lepidoptera pests, the maize expresses Bacillus thuringiensis crystal proteins (delta-endotoxins) Cry1Ab and Cry1F, as well as vegetative insecticidal protein 3Aa20. For resistance to Coleoptera pests, the maize expresses B. thuringiensis modified Cry3A and engineered Cry3.1Ab1. For tolerance to glufinosate, the maize expresses Streptomyces viridochromogenes phosphinothricin N-acetyaltransferase, which inactivates the herbicide through acetylation. For tolerance to glyphosate, the maize expresses a modified maize 5-enolpyruvylshikimate-3-phosphate synthase, which does not bind the herbicidal compound with high affinity. For improved use in downstream biofuel applications, the maize expresses a chimeric Thermococcales sp. alpha amylase, which has increased thermostability and starch hydrolysis activity during the high temperatures in dry-grind ethanol production from maize. The maize also contains Escherichia coli phosphomannose isomerase, which allows for mannose to be utilized as a carbon source and thus was as a selectable marker during transformation of the parental lines.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
-
BCH-LMO-SCBD-15109-9 Living Modified Organism SYN-E3272-5 - Enogen™ MaizeUse in industrial applications (Biofuel production)
-
BCH-LMO-SCBD-15105-12 Living Modified Organism SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maizeResistance to diseases and pests (Insects, Coleoptera (beetles))
-
BCH-LMO-SCBD-14797-15 Living Modified Organism SYN-BTØ11-1 - YieldGard™ maizeResistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis)), Resistance to herbicides (Glufosinate)
-
BCH-LMO-SCBD-14841-13 Living Modified Organism DAS-Ø15Ø7-1 - Herculex™ I maizeDow AgroSciences, Pioneer Hi-Bred International Inc. | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Resistance to herbicides (Glufosinate)
-
BCH-LMO-SCBD-100885-13 Living Modified Organism SYN-IR162-4 - Agrisure™ Viptera maizeResistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
-
BCH-LMO-SCBD-104791-4 Living Modified Organism SYN-Ø53Ø7-1 - Agrisure® Duracade™ MaizeSyngenta Seeds GmbH | Resistance to diseases and pests (Insects, Coleoptera (beetles), Western corn rootworm (Diabrotica virgifera), Northern corn rootworm (Diabrotica barberi))
-
BCH-LMO-SCBD-14794-18 Living Modified Organism MON-ØØØ21-9 - Roundup Ready™ maizeMonsanto | Resistance to herbicides (Glyphosate)
EN
pNOV7013; pZO1502; pNOV1300; pZM26; PHI8999A; pSYN12274; pDPG434
EN
- Cross breeding
|
0.677 kb
|
|
1.383 kb
|
|
0.006 kb
|
|
0.108 kb
|
|
0.070 kb
|
|
1.993 kb
|
|
0.000 kb
|
|
1.176 kb
|
|
0.253 kb
|
|
0.510 kb
|
|
0.470 kb
|
|
1.850 kb
|
|
0.250 kb
|
|
0.420 kb
|
|
0.180 kb
|
|
0.550 kb
|
|
0.250 kb
|
|
1.993 kb
|
|
2.370 kb
|
|
0.108 kb
|
|
0.070 kb
|
|
1.993 kb
|
|
1.176 kb
|
|
0.253 kb
|
|
2.556 kb
|
|
1.797 kb
|
|
0.253 kb
|
|
0.983 kb
|
|
1.010 kb
|
|
1.176 kb
|
|
0.253 kb
|
|
0.980 kb
|
|
1.000 kb
|
|
1.820 kb
|
|
0.720 kb
|
|
0.550 kb
|
|
0.550 kb
|
|
0.200 kb
|
|
0.346 kb
|
|
1.960 kb
|
|
0.253 kb
|
|
1.993 kb
|
|
1.176 kb
|
|
0.253 kb
|
|
1.370 kb
|
|
0.000 kb
|
|
0.370 kb
|
|
1.340 kb
|
|
0.240 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-103622-5 27kD gamma-zein Promoter | Zea mays (Maize, Corn, MAIZE)Promoter
-
BCH-GENE-SCBD-14966-7 amy797E alpha amylase | Thermococcales spp. (Thermococcus)Protein coding sequence | Thermostable alpha-amylase,Use in industrial applications (Biofuel production)
-
BCH-GENE-SCBD-102033-4 SEKDEL ER retention signalTransit signal
-
BCH-GENE-SCBD-101406-4 Phosphoenolpyruvate carboxylase, intron 9 | Zea mays (Maize, Corn, MAIZE)Intron
-
BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
-
BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
-
BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
-
BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | Escherichia coli (ECOLX)Protein coding sequence | Mannose tolerance,Selectable marker genes and reporter genes
-
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
-
BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
-
BCH-GENE-SCBD-103625-3 Alcohol dehydrogenase 1, intron 6 | Zea mays (Maize, Corn, MAIZE)Intron
-
BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
-
BCH-GENE-SCBD-103867-2 Alcohol dehydrogenase 1, intron 2 | Zea mays (Maize, Corn, MAIZE)Intron
-
BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
-
BCH-GENE-SCBD-100887-5 Vegetative insecticidal protein 3Aa20 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
-
BCH-GENE-SCBD-103881-2 Metallothionein-like gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
-
BCH-GENE-SCBD-43634-3 mCry3A | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles), Western corn rootworm (Diabrotica virgifera))
-
BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
-
BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)Terminator
-
BCH-GENE-SCBD-104788-2 Cestrum Yellow Leaf Curling Virus promoter | Cestrum yellow leaf curling virus (CYLCV)Promoter
-
BCH-GENE-SCBD-104789-2 eCry3.1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles), Western corn rootworm (Diabrotica virgifera), Northern corn rootworm (Diabrotica barberi))
-
BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoter
-
BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
-
BCH-GENE-SCBD-101419-4 Optimized Transit PeptideTransit signal
-
BCH-GENE-SCBD-46333-8 5-enolpyruvylshikimate-3-phosphate synthase | Zea mays (Maize, Corn, MAIZE)Protein coding sequence | Resistance to herbicides (Glyphosate)
DNA insert from 3272 vector
The DNA insert in the 3272 genome contains two gene cassettes: Thermococcales sp. alpha amylase (amy797E) and Escherichia coli phosphomannose isomerase (pmi).
The coding sequence of amy797E is under control of the Zea mays (maize) 27kD gamma-zein promoter and Cauliflower mosaic virus 35S terminator. A maize phosphoenolpyruvate carboxylase intron 9 was included to enhance expression of amy797E. A synthetic SEKDEL endoplasmic retention signal was also included to retain the AMY797E protein in the endoplasmic reticulum of cells. Due to the endosperm (seed)-specific nature of the promoter, expression of AMY797E is expected in the endosperm of maize kernels only.
The pmi coding sequence is under control of a maize ubiquitin promoter and Agrobacteirum tumefaciens nopaline synthase terminator. The first intron of the maize ubiquitin sequence was included to enhance expression of the pmi sequence.
Note:
The DNA insert in the 3272 genome contains two gene cassettes: Thermococcales sp. alpha amylase (amy797E) and Escherichia coli phosphomannose isomerase (pmi).
The coding sequence of amy797E is under control of the Zea mays (maize) 27kD gamma-zein promoter and Cauliflower mosaic virus 35S terminator. A maize phosphoenolpyruvate carboxylase intron 9 was included to enhance expression of amy797E. A synthetic SEKDEL endoplasmic retention signal was also included to retain the AMY797E protein in the endoplasmic reticulum of cells. Due to the endosperm (seed)-specific nature of the promoter, expression of AMY797E is expected in the endosperm of maize kernels only.
The pmi coding sequence is under control of a maize ubiquitin promoter and Agrobacteirum tumefaciens nopaline synthase terminator. The first intron of the maize ubiquitin sequence was included to enhance expression of the pmi sequence.
Note:
- The amy797E is a chimeric, thermostable protein that consists of three thermostable amylase enzymes. The sequence was then further codon optimized for expression in maize.
- Southern blot analysis indicated that the 3272 genome contains a single insertion without vector backbone sequences or re-arrangements.
DNA insert from Bt11 vector pZO1502
The DNA insert from the Bt11 genome contains two gene cassettes: Bacillus thuringiensis cry1Ab and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat).
Transcription of cry1Ab gene is under control of the Cauliflower mosaic virus (CaMV) 35S promoter and the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The transcript initially contains Zea mays alcohol dehydrogenase 1 intron 6, which enhance gene expression.
Transcription of pat is under control of the CaMV 35S promoter and nos terminator. The transcript initially contains Zea mays alcohol dehydrogenase 1 intron 2 to enhance gene expression.
Note:
- The CaMV promoter associated with cry1Ab, was isolated from the CM1841 strain of CaMV using DdeI restriction digestion. However, the DdeI sites were converted into SacI sites.
- The CaMV promoter associated with pat was isolated from the Cabb-S strain of CaMV (AluI to DdeI fragment) and subsequently modified.
- The cry1Ab coding sequence encodes a truncated version corresponding to the N-terminal 615 amino acids of the full length Cry1Ab protein.
- The cloning of pat did not result in any amino acid sequence changes in the parental line.
- The nos terminator corresponds to the 423 to 678 basepairs of the nopaline synthase gene in A. tumefaciens.
DNA insert from MIR162 vector pNOV1300
The parental genome contains two gene cassettes: a variant of the native B. thuringiensis vegetative insecticidal protein 3Aa (vip3Aa20) and E. coli phosphomannose isomerase.
Transcription of vip3Aa20 commences at the Z. mays ubiquitin promoter and then transcribes vip3Aa20 followed by intron 9 of Z. mays phosphoenolpyruvate carboxylase, before terminating at the CaMV 35S terminator. Nigher levels of expression are expected due to the promoter and the enhancement by the intron.
The pmi coding sequence is under the control of Z. mays ubiquitin promoter and transcription terminates at the Agrobacterium tumefaciens nopaline synthase gene (nos) terminator.
Note:
- Southern blot analyses demonstrated that the T-DNA insert contains: (i) single copies of a vip3Aa20 gene and a pmi gene; (ii) two copies of the maize ubiquitin promoter; (iii) one copy of the nos terminator; and (iv) no backbone sequences from transformation plasmid pNOV1300.
- In the parental MIR162 maize, a variant of the native B. thuringiensis vegetative insecticidal protein 3Aa (vip3Aa20), named vip3Aa19, which has codon changes that result in a single M129I amino acid substitution was inserted into the transformation cassette. During the transformation process an additional DNA mutation resulted in a K284Q amino acid substitution.
DNA insert from MIR604 vector pZM26
The parental plant contains two expression cassettes: (i) modified Cry3a (mcry3a) originally from Bacillus thuringiensis and (ii) phosphomannose isomerase (pmi) from Escherichia coli.
Expression mcry3a is under control of a Zea mays metallothionein-like gene promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. Transcription of pmi is under the control of Z. mays ubiquitin gene promoter and an A. tumefaciens nos terminator. The transcript initially also contains an intron from Z. mays ubiquitin-1 to enhance gene expression.
Note:
- mcry3a was originally obtained from the native cry3A gene, but was modified to enhance gene expression in maize. The synthetic version of the protein (mCry3a) contains the same amino acid sequences of the native version, except for the modified serine-protease recognition site.
- The following changes in the pmi occurred: the valine at position 61 has been substituted by alanine (V61A) and glutamine at position 210 has been substituted by histidine (Q210H). Please note no apparent change of function occurred.
- Southern blot and qPCR analysis indicated that a single insertion of both expression cassettes occurred and there was no integration of the vector backbone.
DNA insert from TC1507 vector PHI8999A
DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for Bacillus thuringiensis cry1F and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat).
Transcription of cry1F is directed by the promoter and first exon and intron of the maize (Zea mays) ubiquitin gene and terminates at the Agrobacterium tumefaciens ORF25 terminator.
Transcription of the pat gene commences from the CaMV 35S promoter and ends at the CaMV 35S terminator.
Note:
- The coding sequence of both genes has been optimized to achieve a high level of expression in maize.
- The sequences of the complete cry1F and pat are identical to those in the original plasmid.
- The Cry1F protein includes the F604K (phenylalanine to lysine at position 604) amino acid substitution, which was introduced to create a specific restriction site for cloning purposes.
DNA insert from 5307 vector pSYN12274
The DNA insert contains two gene cassettes for an engineered chimeric protein eCry3.1Ab and an Escherichia coli phosphomannose isomerase (pmi).
Transcription of ecry3.1Ab is under control of a Cestrum yellow leaf curling virus promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. Transcription of pmi is under control of a Zea mays ubiquitin gene promoter and a nos terminator. The promoter contains the first intron of the ubiquitin gene, which will be initially included in the mRNA before splicing and for enhancing expression of pmi. Transcription is expected to be constitutive under both promoters and result in elevated levels of transgene expression.
Note:
- eCry3.1Ab is a result of a fusion of the 5′ end (Domain I, Domain II and 15 amino acids of Domain III) of a modified Cry3A gene and the 3′ end (Domain III and Variable Region 6) of a synthetic cry1Ab gene. The sequences were sourced from Bacillus thuringiesis.
- Southern blot analysis indicated that the parental line contains a single insertion of the vector and there was no integration of the vector backbone.
- Sequencing analysis indicated that the right and left T-DNA borders were truncated.
DNA insert from GA21 vector pDPG434
The DNA insert from the GA21 genome contains one gene cassette: Zea mays modified 5-enolpyruvylshikimate-3-phosphate synthase (mepsps).
Transcription of mepsps commences from the Oryza sativa (rice) actin 1 promoter and terminates at the Agrobacterium tumefaciens nopaline synthase terminator. The transcribed elements (from 5’ to 3’) are expected to be as follows: first intron of rice actin 1, a synthetic transit peptide and mepsps. Transcription of mepsps is expected to occur constitutively due to the rice actin promoter. Gene expression is additionally enhanced by the rice actin intron. Post-translation, the optimized transit peptide targets mEPSPS to the chloroplasts.
Note:
- The coding sequence of mepsps was obtained through site-directed mutagenesis to create a modified version of the native enzyme to confer glyphosate tolerance with similar enzymatic function.
- The Rice Actin 1 promoter contains a portion of the first intron of the Actin 1 and thus corresponds to the 5’ end of the gene.
- The optimized transit peptide was derived from maize and sunflower (Helianthus sp.) ribulose 1,5 –bisphosphate carboxylase oxygenase sequences.
- Southern blot analysis indicated that an insert containing three complete tandem copies of the insert and one incomplete copy were inserted into the parental genome. The incomplete copy contains rice actin promoter, the optimized transit peptide and a truncated mepsps sequence without the nos 3’ untranslated region (as uncovered by sequence analysis).
- Sequencing analysis indicated a truncated rice actin promoter in the 5’ end of the insertion event, only containing 148 bp of the promoter region.
- The modified maize expresses only the full-length mEPSPS protein.
EN
- Biofuel
- DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-ØØØ21-9 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-Ø53Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-BTØ11-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-E3272-5 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-IR162-4 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-IR6Ø4-5 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-E3272-5 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- SYN-BTØ11-1 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- SYN-IR162-4 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- SYN-IR6Ø4-5 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- DAS-Ø15Ø7-1 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- SYN-Ø53Ø7-1 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- MON-ØØØ21-9 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
Kindly refer to the parental records for more information.
| Record type | Field | Record(s) | |||||
|---|---|---|---|---|---|---|---|
|
|||||||
