ACS-BNØØ4-7 × ACS-BNØØ3-6 - InVigor™ canola | BCH-LMO-SCBD-101077 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
published: 29 Mar 2010 last updated: 24 Jul 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
InVigor™ canola
EN
MS1(B91-4) x RF3
Yes
ACS-BNØØ4-7 × ACS-BNØØ3-6
The stacked canola line ACS-BNØØ4-7 x ACS-BNØØ3-6 was obtained through the conventional cross breeding of each of the parental organisms.  This results in a line with male-sterility, fertility restoration, pollination control system displaying glufosinate herbicide tolerance.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-9845-4 Organism Brassica rapa (Canola plant)
    Crops
  • BCH-LMO-SCBD-14756-5 Living Modified Organism ACS-BNØØ4-7 - InVigor™ canola
    Bayer CropScience | Changes in physiology and/or production (Reproduction, Male sterility), Resistance to antibiotics (Kanamycin), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-14755-7 Living Modified Organism ACS-BNØØ3-6 - InVigor™ canola This document has been updated. This is not the latest published version. Click here to view the latest version of the record.
    Bayer CropScience | Changes in physiology and/or production (Fertility restoration), Resistance to herbicides (Glufosinate)
EN
  • ACS-BNØØ4-7 × ACS-BNØØ1-4 - InVigor™ canola
    | Bayer CropScience | Changes in physiology and/or production (Reproduction, Male sterility), Resistance to antibiotics (Kanamycin), Resistance to herbicides (Glufosinate)
  • ACS-BNØØ4-7 × ACS-BNØØ2-5 - InVigor™ canola
    | Bayer CropScience | Changes in physiology and/or production (Reproduction, Male sterility), Resistance to antibiotics (Kanamycin), Resistance to herbicides (Glufosinate)
Characteristics of the modification process
pTTM8RE and pTHW118
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-14973-6 Barnase | Bacillus amyloliquefaciens (BACAM)
    Protein coding sequence | Changes in physiology and/or production (Reproduction, Male sterility)
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-14974-7 Barstar | Bacillus amyloliquefaciens (BACAM)
    Protein coding sequence | Changes in physiology and/or production (Fertility restoration)
  • BCH-GENE-SCBD-101407-6 pTA29 pollen specific promoter | Nicotiana tabacum (Tobacco, TOBAC )
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-101902-4 rbcS Transit Peptide | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101409-2 Barstar gene terminator | Bacillus amyloliquefaciens (BACAM)
    Terminator
DNA insert from ACS-BNØØ4-7 vector pTTM8RE
ACS-BNØØ4-7 is a male-sterile canola line that cannot produce viable pollen due to the presence of the barnase gene. The line also contributes a copy of the bar gene which confers tolerance to the herbicide glufosinate.

DNA insert from ACS-BNØØ3-6 vector pTHW118
ACS-BNØØ3-6 is a fertility restorer canola line that inhibits the action of the barnase ribonuclease through the expression of the barstar gene. The line also contributes a copy of the bar gene which confers tolerance to the herbicide glufosinate.

For additional information on this LMO, please refer to the records of the parental LMOs.
EN
LMO characteristics
EN
  • Food
  • Feed
Additional Information
The transgenic line MS1 (B91-4) was produced by genetically engineering plants to be male sterile and tolerant to the herbicide glufosinate ammonium (as a selectable marker). The parental line MS1 contains the barnase gene for male sterility, isolated from Bacillus amyloliquefaciens, a common soil bacterium that occurs naturally in the soil and in various organisms including bacteria and plants, and frequently used as a source for industrial enzymes. The barnase gene encodes for a ribonuclease enzyme (RNAse) expressed only in the tapetum cells of the pollen sac during anther development. The RNAse affects RNA production, disrupting normal cell functioning and arresting early anther development, thus leading to male sterility.

The transgenic line RF3 (DBN212-0005) was produced by genetically engineering plants to restore fertility in the hybrid line and to be tolerant to the herbicide glufosinate ammonium (as a selectable marker). Transgenic RF3 plants contain the barstar gene isolated from Bacillus amyloliquefaciens. The barstar gene codes for a ribonuclease inhibitor (barstar enzyme) that is expressed only in the tapetum cells of the pollen sac during anther development. The ribonuclease inhibitor specifically inhibits barnase RNAse expressed by the MS8 line. Together, the RNAse and the ribonuclease inhibitor form a very stable one-to-one complex, in which the RNAse is inactivated. As a result, when pollen from the restorer line RF3 is transferred to the male sterile line MS8, the resultant progeny express the RNAse inhibitor in the tapetum cells of the anthers allowing hybrid plants to develop normal anthers and restore fertility.
EN
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