ACS-BNØØ5-8 × MON-ØØØ73-7 - Male-sterile, Herbicide-tolerant canola | BCH-LMO-SCBD-116284 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
Decisions on the LMO Risk Assessments  
last updated: 27 Sep 2021
Living Modified Organism identity
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Male-sterile, Herbicide-tolerant canola
MS8 × RT73
The modified canola (Brassica napus) was produced through cross breeding of previously modified parental lines for male sterility and herbicide tolerance. The male tissue specific expression of Streptomyces hygroscopicus barnase, disrupts pollen development and results in male sterility. For herbicide tolerance, the modified canola expresses Streptomyces hygroscopicus phosphinothricin acetyltransferase (glufosinate tolerance), Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (glyphosate tolerance) and Ochrobactrum anthropi glyphosate oxidoreductase (glyphosate tolerance).
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-12083-7 Organism Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)
  • BCH-LMO-SCBD-14759-8 Living Modified Organism ACS-BNØØ5-8 - InVigor™ canola
    Bayer CropScience | Changes in physiology and/or production (Reproduction, Male sterility), Resistance to antibiotics (Kanamycin), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-14795-11 Living Modified Organism MON-ØØØ73-7 - Roundup Ready™ canola
    Monsanto | Resistance to herbicides (Glyphosate)
Characteristics of the modification process
pTHW107; PV-BNGT04
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-101407-6 pTA29 pollen specific promoter | Nicotiana tabacum (Tobacco, TOBAC )
  • BCH-GENE-SCBD-14973-6 Barnase | Bacillus amyloliquefaciens (BACAM)
    Protein coding sequence | Changes in physiology and/or production (Reproduction, Male sterility)
  • BCH-GENE-SCBD-104825-2 Barnase 3' Untranslated region | Bacillus amyloliquefaciens (BACAM)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
  • BCH-GENE-SCBD-101902-4 rbcS Transit Peptide | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-14998-4 Glyphosate oxidoreductase gene | Ochrobactrum anthropi (OCHAN)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
DNA insert from pTHW107 from MS8 (ACS-BNØØ5-8) canola
The DNA insert contains two cassettes: Bacillus amyloliquefaciens barnase and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar).

Barnase is under control of a Nicotiana tabacum TA29 pollen specific promoter and an Agrobacterium tumefaciens nopaline synthase terminator. An additional sequence, B. amyloliquefaciens barnase 3’ untranslated region, which contributes to the polyadenylation of the coding sequence, can be found between the barnase coding sequence and nopaline synthase terminator.

Phosphinothricin N-acetyltransferase is under control of an Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunit promoter and A. tumefaciens transcript 7 3’ untranslated region.

- The coding sequence of bar has the two N-terminal codons modified to ATG and GAC.
- Southern blot and PCR analysis indicated that a single intact copy of the transformation cassette was integrated into the parental genome.

DNA insert from PV-BNGT04 from RT73 (MON-ØØØ73-7) canola
The DNA insert contains two gene cassettes: Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps) and Ochrobactrum anthropic glyphosate oxidoreductase (gox).

The cp4-epsps coding sequence is under control of a Figwort mosaic virus 34S promoter and a Pisum sativum rubisco small subunit terminator. A transit peptide from ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunit was included before the cp4-epsps coding sequence. The transit peptide leads to high-level of expression in leaf tissues.

- The size of the genetic elements were not available at time of this record's creation
- The gox coding sequence differs from the wild-type version of the gene at 3 amino acid sites (G85S, R153K and R334H) and was designated as goxv247.
- The cp4-epsps sequence was optimized for expression in plants.
- PCR and southern blot analyses indicated that the parental genome contains a single insertion event containing one copy of the T-DNA from plasmid PV-BNGT04. No genetic elements from outside of the right and left borders of the plasmid were transferred into or are present in the genomic DNA of the LMO.

For more information, kindly refer to the parental LMO records.
LMO characteristics
  • Food
  • Feed
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1