KM-ØØØH71-4 - Roundup Ready™ sugar beet | BCH-LMO-SCBD-14773 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 21 Oct 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ sugar beet
H7-1 sugar beet was modified with the insertion of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) from Agrobacterium tumefaciens, thereby conferring tolerance to the herbicide glyphosate.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
The nucleic acid sequence of the epsps gene was optimised for expression in plants.
LMO characteristics
  • Food
Additional Information
Both the endogenous EPSPS and the EPSPS introduced into the sugar beet plants by means of transformation catalyse the reaction of shikimate-3-phosphate with phosphoenolpyruvate to yield 5-enolpyruvylshikimate-3-phosphate, an intermediate stage in the biosynthesis of aromatic amino acids. In contrast to the endogenous EPSPS, the EPSPS inserted into the genetically modified sugar beet plants is not inhibited by glyphosate. The upstream position of the transit peptide CTP2 of the EPSPS derived from Arabidopsis thaliana causes the post-translational import of the chimeric protein into the chloroplasts.