DAS-24236-5 × DAS-21Ø23-5 × MON-88913-8 - Widestrike™ Roundup Ready Flex™ cotton | BCH-LMO-SCBD-15169 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
published: 20 Jun 2006 last updated: 12 Feb 2018
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Widestrike™ Roundup Ready Flex™ cotton
EN
281-24-236 x 3006-210-23 x MON88913 x DAS-81910-7
Yes
DAS-24236-5 × DAS-21Ø23-5 × MON-88913-8
A stacked insect-resistant and glyphosate-tolerant cotton derived from conventional cross-breeding of DAS-21Ø23-5 x DAS-24236-5 with MON-88913-8.  Inserted genes include the cry1Ac gene from Bacillus thuringiensis subsp. kurstaki, the cry1F gene from Bacillus thuringiensis var. aizawai, the PAT encoding gene from Streptomyces viridochromogenes, and a naturally glyphosate tolerant form of the enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) from Agrobacterium tumefaciens strain CP4 to confer tolerance to phosphinothricin (glyphosate) herbicide.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)
    Crops
  • BCH-LMO-SCBD-15168-16 Living Modified Organism MON-88913-8 - Roundup Ready™ Flex™ cotton
    Monsanto | Resistance to herbicides (Glyphosate)
  • BCH-LMO-SCBD-14938-7 Living Modified Organism DAS-21Ø23-5 - Insect-resistant cotton
    Dow AgroSciences | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-LMO-SCBD-14940-7 Living Modified Organism DAS-24236-5 - Insect-resistant cotton
    Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
EN
Characteristics of the modification process
pAGM281, PV-GHGT35 and pMYC3006
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
    Promoter
  • BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)
    Intron
  • BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-103900-3 4ocs∆Mas2' promoter
    Promoter
  • BCH-GENE-SCBD-103903-1 Elongation factor EF-1alpha promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-103904-1 Elongation factor EF-1alpha Leader | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Leader
  • BCH-GENE-SCBD-103905-1 Elongation factor EF-1alpha Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Intron
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
    Terminator
  • BCH-GENE-SCBD-103907-3 Actin 8 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-103908-4 Actin 8 Leader sequence | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Leader sequence
  • BCH-GENE-SCBD-103909-3 Actin 8 Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Intron
  • BCH-GENE-SCBD-105196-2 FMV 35S Enhancer | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
    Leader
  • BCH-GENE-SCBD-105197-2 CaMV 35S Enhancer | Cauliflower mosaic virus (CaMV)
    Leader
DNA insert from DAS-21Ø23-5, vector pMYC3006:
Lepidopteran-resistant cotton, with insect resistance conferred by the cry1Ac gene from the common soil bacterium Bacillus thuringiensis (Bt) var. kurstaki strain HD73. Also contains a coding sequence or the Phosphinothricin N-acetyltransferase gene which confers tolerance to glufosinate.

DNA insert from DAS-24236-5, vector pAGM281:
Lepidopteran-resistant cotton, with insect resistance conferred by the cry1F gene from the common soil bacterium Bacillus thuringiensis (Bt) var. aizawai. Also contains a coding sequence or the Phosphinothricin N-acetyltransferase gene which confers tolerance to glufosinate.

DNA insert from MON-88913-8, vector PV-GHGT35:
Roundup Ready® Flex cotton (MON 88913) was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option in cotton production. The transforming plasmid PV-GHGT35 carried a transfer DNA sequence comprising of two 5-enolpyruvylshikimate-3-phosphate synthase gene cassettes.

For additional information on this LMO, please refer to the records of the parental LMOs.
EN
LMO characteristics
EN
  • Fiber/textile
Additional Information
DAS-21Ø23-5 x DAS-24236-5 x MON-88913-8 was produced by crossing WideStrike™ insect resistant cotton (DAS-21Ø23-5 x DAS-24236-5) with the herbicide-tolerant cotton line MON-88913-8. This stacked cotton line expresses four novel proteins: the delta-endotoxins Cry1F and Cry1Ac, which confer resistance to the lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm, the CP4 EPSPS protein, which confers tolerance to the herbicide glyphosate, and the PAT protein, which confers tolerance to glufosinate ammonium. The insecticidal proteins Cry1F and Cry1Ac are produced by the cry1F gene and cry1Ac genes respectively, both of which are from WideStrike™. The cotton line WideStrike™ was produced by crossing line 281-24-236 with line 3006-210-23. The PAT protein in WideStrike™ is produced by the pat gene: this expressed novel protein was intended solely for use as a selectable marker during plant transformation. The CP4 EPSPS protein is produced by the cp4 epsps gene from MON-88913-8.

This stacked cotton line is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.
EN
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 6
Country's Decision or any other Communication Living modified organism(s) 6