IND-1ØØ15-7 - Chymosin-producing safflower | BCH-LMO-SCBD-259218 | Living Modified Organism | Biosafety Clearing-House

Loading...
  |  

Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 11 Feb 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Chymosin-producing safflower
EN
IND-10015-7
Yes
IND-1ØØ15-7
  • loading record details...
The safflower (Carthamus tinctorius L.) was modified for the production of Bos taurus chymosin in seed tissues. The enzyme is used in the production of cheese products to coagulate (clot) milk. In addition to the production of the enzyme, the safflower expresses Streptomyces viridochromogenes phosphinothricin N-acetyaltransferase for glufosinate selection during transformation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • loading record details...
EN
Characteristics of the modification process
pSBS2165
EN
 
0.000 kb
 
 
0.000 kb
 
 
0.000 kb
 
 
0.000 kb
 
 
0.000 kb
 
 
0.000 kb
 
 
0.000 kb
 
 
0.000 kb
 
 
0.000 kb
 
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • loading record details...
  • loading record details...
  • loading record details...
  • loading record details...
  • loading record details...
  • loading record details...
  • loading record details...
  • loading record details...
  • loading record details...
The modified safflower contains two gene cassettes: Bos taurus chymosin (cym) and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat).

The cym coding sequence is under control of the Phaseolus vulgaris phaseolin promoter and terminator. The coding sequence additionally contains a pathogenesis-related protein R transit peptide, which will direct the CYM protein to the apoplast of seed tissues. Expression of the cym coding sequence is expected to be restricted to seed tissues.

The pat coding sequence is under control of the Petroselinum crispum ubiquitin promoter and terminator. Due to the constitutive nature of the promoter, high levels are transcription are expected. 

Note:
  • Southern blot indicated that the genome contain a single T-DNA insertion.
  • Sequencing and PCR analyses indicated that the sequences are intact and without rearrangements.
  • Sequencing analysis additionally indicated that no vector backbone sequences were integrated into the safflower genome.
EN
LMO characteristics
EN
Detection method(s)
EN
Additional Information
EN
Loading...