BCS-GHØØ2-5 - GlyTol™ Cotton GHB614 | BCH-LMO-SCBD-46334 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
Decisions on the LMO Risk Assessments  
published: 13 Aug 2008 last updated: 28 Jan 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
GlyTol™ Cotton GHB614
  • - Person: Bayer CropScience | BCH-CON-CA-7708-1
    Bayer CropScience
GlyTol cotton event GHB614 contains a stably integrated gene 2mepsps. The 2mepsps gene was generated by introducing mutations into the wild-type epsps (wt epsps) gene from maize, leading to a double mutant EPSPS protein with two amino acid substitutions (2mEPSPS). This modification confers the protein a decreased binding affinity for glyphosate, allowing it to maintain sufficient enzymatic activity in the presence of the herbicide. Therefore, the plants bearing this gene are tolerant to glyphosate herbicides.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Variety: Coker 312
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-46333-8 5-enolpyruvylshikimate-3-phosphate synthase | Zea mays (Maize, Corn, MAIZE)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-101419-4 Optimized Transit Peptide
    Transit signal
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-104647-3 Histone H4 gene Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-104648-2 Histone H3 Gene II intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-104646-4 Histone H4 gene 3' UTR | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
Southern blot analysis indicated that a single insertion of the complete T-DNA as constructed occurred in the GHB614 line. There was no indication that any portion of the vector backbone also integrated into the GHB614 line.
LMO characteristics
  • Food
  • Feed
  • Fiber/textile
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 8
Country's Decision or any other Communication Living modified organism(s) 22
Risk Assessment generated by a regulatory process Living modified organism(s) 23
Living Modified Organism Related LMO(s) 1
Living Modified Organism Recipient Organism” or “Parental Organisms 13
Risk Assessment generated by an independent or non-regulatory process Living modified organism(s) 1