BCS-OSØØ3-7 - Liberty Link™ rice | BCH-LMO-SCBD-47517 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 21 Nov 2008 last updated: 06 May 2013
Living Modified Organism identity
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Liberty Link™ rice
Rice resistant to the herbicide glufosinate through introduction of the bar gene from Streptomyces hygroscopicus which encodes the enzyme phosphinothricin-N-acetyltransferase (PAT) that catalyzes the acetylation of phosphinothricin (glufosinate), detoxifying it into an inactive compound.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Variety: Cocodrie
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
The phosphinothricin-N-acetyltransferase enzyme coding sequence was obtained from the Streptomyces hygroscopicus HP632 strain. the The 2 N-terminal codons of the wild type gene were modified to an ATG and GAC respectively.

Southern blot analysis indicated the presence of a single bar gene flanked its regulatory genetic elements. further analysis indicated the the 3' nos terminator was truncated during the transformation process. Additionally a second copy of the 35S promoter or part of it was inserted into the host genome.

Bioinformatic analysis by the company of the regions flanking the bar gene suggests that no known rice gene was interrupted and that no chimeric proteins would be expressed due to the gene insertion. Such an analysis was not performed for the second insertion containing 35S promoter sequences.
LMO characteristics
  • Food
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 8
Country's Decision or any other Communication Living modified organism(s) 2
Risk Assessment generated by a regulatory process Living modified organism(s) 1
Living Modified Organism Related LMO(s) 2