DP-Ø9814Ø-6 - Optimum™ GAT™ maize | BCH-LMO-SCBD-48466 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
Decisions on the LMO Risk Assessments  
published: 25 Mar 2009 last updated: 20 Mar 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Optimum™ GAT™ maize
The 98140 maize has been genetically modified (GM) to express the GAT4621 and ZM-HRA proteins. The GAT4621 protein is a glyphosate acetyltransferase (GAT), encoded by an optimized form of the gat gene from Bacillus licheniformis. When cultivated, expression of the GAT4621 protein in 98140 maize confers tolerance to the herbicide glyphosate. The ZM-HRA protein is an acetolactate synthase (ALS) encoded by the zm-hra gene, an optimized form of the endogenous als gene from Zea mays. When cultivated, expression of the ZM-HRA protein in 98140 maize confers tolerance to ALS-inhibiting herbicides, such as chlorimuron and thifensulfuron.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
For transformation of 98140 maize, binary vector PHP24279 was used. The between the left and right border repeats, the inserted T-DNA consists of a 7440 bp sequence contains, in addition to the genetic elements listed above:

Three copies of the CaMV 35S enhancer region from cauliflower mosaic virus between the promoters of the two expression casettes which contribute to enhance expression of both the gat4621 and zm-hra genes.
LMO characteristics
  • Food
  • Feed
Detection method(s)
The results of the molecular characterization described in this application support the conclusion that 98140 maize contains a single and full-length copy of the T-DNA region from binary vector PHP24279.
Additional Information
Authorisation of import and processing of DP-Ø9814Ø-6 in the EU has been applied for.