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Modified Organism
SYN-IR162-4 - Agrisure™ Viptera maize
Record information and status
Record ID
100885
Status
Published
Date of creation
2009-11-26 20:22 UTC (pafca@mre.gov.br/7317)
Date of last update
2017-06-05 15:08 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-06-05 15:08 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Agrisure™ Viptera maize
Transformation event
MIR162
Unique identifier
SYN-IR162-4
Developer(s)
Syngenta Crop Protection AG
Schwarzwaldallee 215
Basel
Switzerland, CH 4058
Phone:+41 61 323-1111
Url:Syngenta
Description
MIR162 maize is transformed with vip3Aa20 gene which encodes the Vip3Aa20 protein that confers resistance against lepidopteran insect pests  including: fall armyworm, armyworm , beet armyworm , corn earworm , black cutworm , and western bean cutworm. Event MIR162 maize also contains the manA gene from Escherichia coli, which encodes the selectable marker, phosphomannose isomerase (PMI).

Vip3A is a group of vegetative insecticidal proteins (i.e., produced during the vegetative stage of bacterial growth) from Bacillus thuringiensis. The vip3Aa20 form inserted into MIR162 is a variant of the native vip3Aa gene, which was isolated from Bt strain AB88.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
pNOV1300
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ubiquitin gene promoter
1.99 Kb
 
 
Vegetative insecticidal protein 3Aa20
2.37 Kb
 
 
Phosphoenolpyruvate carboxylase, intron 9
0.11 Kb
 
 
CaMV 35S terminator
0.07 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
In MIR162 maize, a variant of the native Bt Vip3Aa, named vip3Aa19, which has codon changes that result in a single  M129I amino acid substitution was inserted into the transformation cassette. During the transformation process an additional DNA mutation resulted in a K284Q amino acid substitution. This final form was designated the name Vip3Aa20.

The pNOV1300 vector region between the left and right border sequences, which included the vip3Aa19 and pmi gene expression cassettes, was inserted into the maize genome during transformation.

Southern blot analyses demonstrated that the T-DNA insert contains: i) single copies of a vip3Aa20 gene and a pmi gene; ii) two copies of the ZmUbiInt promoter; iii) one copy of the NOS terminator; and iv) no backbone sequences from transformation plasmid pNOV1300.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information

Records referencing this document (66)
IDDescription
66record(s) found
Country's Decision or any other Communication17 records
Modified Organism21 records
Organization9 records
Risk Assessment19 records