Table of Contents: 1. Introductory Background 1.1 Brief overview of the requirements under the Cartagena Protocol on Biosafety 1.2 Brief overview of the requirements under the Nagoya – Kuala Lumpur Supplementary Protocol 1.3 Implementation by Parties 2. National Legislative Process 2.1 Inclusive and participatory process 2.2 Legal Assessment Report 2.3 Legislative solutions 2.4 Administrative tasks 3. Legal Diagnostic Tool 3.1 Background 3.2 Using the Tool 3.3 Target audience 3.4 Inclusions, exclusions and limitations Annex I: Legal Diagnostic Tool

Abstract: Glyphosate, a broad-spectrum herbicide targeting 5-enolpyruvylshikimate-3-phosphate synthase, was first commercialized in 1974. Its use increased significantly following the introduction of glyphosate-resistant (GR) crops in 1996 and the expiration of its patent in 2000, making it the most used herbicide worldwide. Intensive reliance on glyphosate has selected for GR weed populations, with the first documented case reported also in 1996. Glyphosate resistance now has evolved in 62 weed species across 31 countries, often involving multiple other herbicide modes of action and thereby complicating chemical control. The highest incidence has occurred in regions with extensive GR crop cultivation, particularly the United States, Brazil, and Argentina, where glyphosate has been used for years as the sole or major herbicide. Apparent disparities among countries largely reflect differences in the timing and reporting of first unique resistance cases, rather than actual differences in the overall prevalence of GR weeds. GR weeds possess the greatest diversity of resistance mechanisms described for any herbicide, encompassing both target-site and non-target-site resistance mechanisms, frequently in combination. In many cases, resistance traits that provide low resistance levels have combined over time to provide more robust resistance (creeping resistance). Evolution and spread of glyphosate resistance are influenced by frequency of use, herbicide dose, phenological stage, pollination biology, gene flow, and dissemination of GR seeds or propagules via contaminated seed/grain lots and machinery. Glyphosate resistance and controversies regarding environmental and health issues have so far not resulted in marked reductions in glyphosate use nor in the introduction of a herbicide or other technology that is as effective and economical as glyphosate in managing weeds. This review synthesizes current knowledge of glyphosate resistance and its mechanisms, highlighting existing gaps, and discusses potential scenarios for the future of glyphosate and GR crops. © 2026 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Author summary: Living modified organisms containing engineered gene drives (EGD-LMOs) are designed to spread genetic modifications through wild populations and persist – a novel technology that brings with it fresh biosafety challenges. In light of these concerns, a set of guidance materials to support risk assessment of EGD-LMOs has been drawn up by a group of experts and welcomed by Parties to the Cartagena Protocol on Biosafety. However, the guidance materials advocate a narrow approach under which only what are identified as “plausible pathways to harm” are selected for assessment. This methodology fails to sufficiently address the central risks of gene drives arising from their very design objective – spread and persistence. Instead of being limited in scope, risk assessment should cast the net widely to capture all potential harms of EGD-LMOs. Only then will it align with the precautionary principle that is enshrined in the Cartagena Protocol and that will ensure adequate protection from the possible adverse effects of this controversial technology on biodiversity and human health.

Author's Abstract. Eucalyptus comprises the largest planted area of cultivated production forest in Brazil. Genetic modification (GM) of eucalyptus can provide additional characteristics for increasing productivity and protecting wood yield, as well as potentially altering fiber for a diversity of industrial uses. However, prior to releasing a new GM plant, risk assessments studies with non-target organisms must be undertaken. Bees are prominent biological models since they play an important role in varied ecosystems, including for Eucalyptus pollination. The main goal of this study was to evaluate whether a novel event (Eucalyptus 751K032), which carries the cp4-epsps gene that encodes the protein CP4-EPSPS and nptII gene that encodes the protein NPTII, might adversely affect honey bees (Apis mellifera) and stingless bees (Scaptotrigona bipunctata). The experiments were performed in southern Brazil, as follows: (i) larvae and adults were separately investigated, (ii) three or four different pollen diets were offered to bees, depending on larval or adult status, and (iii) two biological attributes, i.e., survivorship of larvae and adults and food intake by adults were evaluated. The diets were prepared with pollen from GM Eucalyptus 751K032; pollen from conventional Eucalyptus clone FGN-K, multifloral pollen or pure larval food. The insecticide dimethoate was used to evaluate the sensitivity of bees to toxic substances. Datasets were analyzed with Chi-square test, survival curves and repeated measures ANOVA. Results indicated no evidence of adverse effects of Eucalyptus pollen 751K032 on either honey bees or stingless bees assessed here. Therefore, the main findings suggest that the novel event may be considered harmless to these organisms since neither survivorship nor food consumption by bees were affected by it.