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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Brassica napus modified for the synthesis of resveratrol and reduced sinapine content
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pPSty5 / pLH-BnSGT-GUS (line 1502.15.7)
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Organization:FINAB e. V. (outdated) ()OtherThünenplatz 1Groß Lüsewitz, Mecklenburg-Vorpommern
18190, GermanyPhone:Fax:Email: webmaster@finab.de,Website: http://www.finab.de,
The aim is to reduce the sinapine content and at the same time to synthesise resveratrol in the GM plants by combining the suppression cassette for the UDP-glucose:sinapate glucosyltransferase (SGT) gene with the gene that encodes the stilbene synthase VST I. In order to achieve this, the oilseed rape plants were co-transformed using the constructs pPSty5 and pLH-BnSGT-GUS.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12083-7 Organism Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)Crops
Cultivar/breeding line: Drakkar, Lisora
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Brassica napus modified for reduced sinapine content (pLH-BnSGT-GUS)| Vereins zur Förderung Innovativer und Nachhaltiger AgroBiotechnologie Mecklenburg-Vorpommern e. V.(FINAB e. V.) | Changes in quality and/or metabolite content (Protein and amino acids), Resistance to herbicides (Glufosinate), Selectable marker genes and reporter genes
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Brassica napus modified for the synthesis of resveratrol| Vereins zur Förderung Innovativer und Nachhaltiger AgroBiotechnologie Mecklenburg-Vorpommern e. V.(FINAB e. V.) | Changes in quality and/or metabolite content (Antioxidants, Flavonoids (e.g. anthocyanin)), Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Fungi)
pPSty5 and pLH-BnSGT-GUS
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101520-4 Stilbene Synthase | Vitis vinifera (Grape Vine)Protein coding sequence | Changes in quality and/or metabolite content (Antioxidants, Flavonoids (e.g. anthocyanin)),Resistance to diseases and pests (Fungi),Tolerance to abiotic stress
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-101523-2 UDP-glucose:sinapate glucosyltransferase | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-101521-6 Napin gene promoter | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103887-1 Stilbene Synthase Terminator | Vitis vinifera (Grape Vine)Terminator
Information Regarding pPSty5
The plasmid used to transform the oilseed rape plants with the pPSty5 construct is derived from the binary vector pPZP111 (Hajdukiewicz et al., 1994) and contains the following genetic elements outside the border regions:
- a bacterial chloramphenicol acetyltransferase gene (CmR gene, cat-Gen), which confers resistance to the antibiotic chloramphenicol;
- the bom sequence from pBR322 for mobilisation of the plasmid from E. coli in Agrobacterium tumefaciens;
- the origins of replication from ColE1 and pVS1 for replication in E. coli or Agrobacterium.
Information Regarding pLH-BnSGT-GUS
The plasmid used to transform the oilseed rape plants with the construct pLH-BnSGT-GUS and is derived from the binary vector pLH7000 (Hausmann and Töpfer, 1999) and contains the following genetic elements outside the border regions:
- the aadA gene for resistance to the antibiotics streptomycin and spectinomycin;
- the bom sequence and the nic sequence from pBR322 for mobilisation of the plasmid from E. coli in Agrobacterium tumefaciens;
- the origins of replication from ColE1 and pVS1 for replication in E. coli or Agrobacterium.
As a rule, in Agrobacterium-mediated transformations only DNA fragments located between the border regions are integrated into the plant genome. However, in isolated cases the transfer of DNA fragments located outside the border regions has been reported and cannot be ruled out entirely.
-RNAi construct: 212 bp fragments of SGT-gen in sense and antisense orientation, seperated by a spacer (nt790-nt1812 of uidA)
EN
The plasmid used to transform the oilseed rape plants with the pPSty5 construct is derived from the binary vector pPZP111 (Hajdukiewicz et al., 1994) and contains the following genetic elements outside the border regions:
- a bacterial chloramphenicol acetyltransferase gene (CmR gene, cat-Gen), which confers resistance to the antibiotic chloramphenicol;
- the bom sequence from pBR322 for mobilisation of the plasmid from E. coli in Agrobacterium tumefaciens;
- the origins of replication from ColE1 and pVS1 for replication in E. coli or Agrobacterium.
Information Regarding pLH-BnSGT-GUS
The plasmid used to transform the oilseed rape plants with the construct pLH-BnSGT-GUS and is derived from the binary vector pLH7000 (Hausmann and Töpfer, 1999) and contains the following genetic elements outside the border regions:
- the aadA gene for resistance to the antibiotics streptomycin and spectinomycin;
- the bom sequence and the nic sequence from pBR322 for mobilisation of the plasmid from E. coli in Agrobacterium tumefaciens;
- the origins of replication from ColE1 and pVS1 for replication in E. coli or Agrobacterium.
As a rule, in Agrobacterium-mediated transformations only DNA fragments located between the border regions are integrated into the plant genome. However, in isolated cases the transfer of DNA fragments located outside the border regions has been reported and cannot be ruled out entirely.
-RNAi construct: 212 bp fragments of SGT-gen in sense and antisense orientation, seperated by a spacer (nt790-nt1812 of uidA)
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- Research
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