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The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
6.2 kb linear fragment PvuII fragment derived from plasmid pAC321
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Acetohydroxy acid synthase gene - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
Resistance to herbicides - Imidazolinone, Sulfonylurea
The csr1-2 coding sequence from Arabisopsis thaliana is
2013 bp long and includes the S653N point mutation which confers
tolerance to imidazolinone herbicides.
In CV127, transcription is directed by the 5' and 3' untranslated
regions (UTR) containing the putative promoter and terminator
regions, respectively, of the csr1-2 gene from Arabidopsis
In addition to the S653N mutation found in the donor organism, DNA
sequence analysis revealed that the csr1-2 gene cassette contains
three point mutations relative to the PvuII linear DNA fragment of
pAC321. One of the point mutations is a G to A mutation at position
272 in the csr1-2 gene, which results in an amino acid change from
arginine to lysine. The other two mutations are genetically
Sequence analysis revealed that parts of the PvuII transformation
fragment are not contained within the transgene insert in CV127.
Deletions of unannotated Arabidopsis genomic DNA occurred both at
the 5' end and 3' end during insertion into the soybean
- Resistance to herbicides
Description of the methods used for the genetic
A purified, linear DNA fragment derived from plasmid pAC321 was
transform embryogenic axis tissue derived from the apical meristem
of a single soybean seed of the commercial variety Conquista using
particle bombardment. No carrier DNA was used in the process.
Nature and source of the vector used
Soybean tissues were transformed with an approximately 6.2 kb
linear fragment PvuII fragment derived from plasmid pAC321
containing the csr1-2 gene cassette.