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Modified Organism
ACS-BNØØ4-7 x ACS-BNØØ3-6 - InVigor™ canola
Record information and status
Record ID
101077
Status
Published
Date of creation
2010-03-29 20:20 UTC (manoela.miranda@cbd.int)
Date of last update
2013-07-24 16:30 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 16:30 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
InVigor™ canola
Transformation event
MS1(B91-4) x RF3
Unique identifier
ACS-BNØØ4-7 x ACS-BNØØ3-6
Developer(s)
Bayer CropScience (Aventis CropScience (AgrEvo))
Bayer CropScience AG
Alfred-Nobel-Str. 50
Monheim am Rhein
D-40789
Germany
Phone:+49-(0)2173-380
Url:http://www.bayercropscience.com
Description
The stacked canola line ACS-BNØØ4-7 x ACS-BNØØ3-6 was obtained through the conventional cross breeding of each of the parental organisms.  This results in a line with male-sterility, fertility restoration, pollination control system displaying glufosinate herbicide tolerance.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica rapa - Canola plant
ACS-BNØØ4-7 - InVigor™ canola
Changes in physiology and/or production - Reproduction - Male sterility Resistance to antibiotics - Kanamycin Resistance to herbicides - Glufosinate
Show detection method(s)
ACS-BNØØ3-6 - InVigor™ canola
Changes in physiology and/or production - Fertility restoration Resistance to herbicides - Glufosinate
Show detection method(s)
Related LMOs
ACS-BNØØ4-7 x ACS-BNØØ1-4 - InVigor™ canola
Changes in physiology and/or production - Reproduction - Male sterility Resistance to antibiotics - Kanamycin Resistance to herbicides - Glufosinate
Show detection method(s)
ACS-BNØØ4-7 x ACS-BNØØ2-5 - InVigor™ canola
Changes in physiology and/or production - Reproduction - Male sterility Resistance to antibiotics - Kanamycin Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
pTTM8RE and pTHW118
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
pTA29 pollen specific promoter
1.50 Kb
 
 
Barnase
0.34 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
rbcS Promoter
1.84 Kb
 
 
rbcS Transit Peptide
0.16 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.50 Kb
 
 
Transcript 7 gene 3' untranslated region
0.20 Kb
 
 
Nopaline Synthase Gene Promoter
0.40 Kb
 
 
Neomycin Phosphotransferase II
1.00 Kb
 
 
Octopine Synthase Gene Terminator
0.90 Kb
 
 
pTA29 pollen specific promoter
1.51 Kb
 
 
Barstar
0.27 Kb
 
 
Barstar gene terminator
0.04 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
rbcS Promoter
1.73 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Transcript 7 gene 3' untranslated region
0.21 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from ACS-BNØØ4-7 vector pTTM8RE
ACS-BNØØ4-7 is a male-sterile canola line that cannot produce viable pollen due to the presence of the barnase gene. The line also contributes a copy of the bar gene which confers tolerance to the herbicide glufosinate.

DNA insert from ACS-BNØØ3-6 vector pTHW118
ACS-BNØØ3-6 is a fertility restorer canola line that inhibits the action of the barnase ribonuclease through the expression of the barstar gene. The line also contributes a copy of the bar gene which confers tolerance to the herbicide glufosinate.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The transgenic line MS1 (B91-4) was produced by genetically engineering plants to be male sterile and tolerant to the herbicide glufosinate ammonium (as a selectable marker). The parental line MS1 contains the barnase gene for male sterility, isolated from Bacillus amyloliquefaciens, a common soil bacterium that occurs naturally in the soil and in various organisms including bacteria and plants, and frequently used as a source for industrial enzymes. The barnase gene encodes for a ribonuclease enzyme (RNAse) expressed only in the tapetum cells of the pollen sac during anther development. The RNAse affects RNA production, disrupting normal cell functioning and arresting early anther development, thus leading to male sterility.

The transgenic line RF3 (DBN212-0005) was produced by genetically engineering plants to restore fertility in the hybrid line and to be tolerant to the herbicide glufosinate ammonium (as a selectable marker). Transgenic RF3 plants contain the barstar gene isolated from Bacillus amyloliquefaciens. The barstar gene codes for a ribonuclease inhibitor (barstar enzyme) that is expressed only in the tapetum cells of the pollen sac during anther development. The ribonuclease inhibitor specifically inhibits barnase RNAse expressed by the MS8 line. Together, the RNAse and the ribonuclease inhibitor form a very stable one-to-one complex, in which the RNAse is inactivated. As a result, when pollen from the restorer line RF3 is transferred to the male sterile line MS8, the resultant progeny express the RNAse inhibitor in the tapetum cells of the anthers allowing hybrid plants to develop normal anthers and restore fertility.

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism2 records