Modified fatty acid, herbicide-tolerant soybean

The stacked soy line DP-3Ø5423-1 x MON-Ø4Ø32-6 was obtained through the traditional cross breading of each of the parental organisms to produce a soy that expresses each of EPSPS, ALS and omega-6-fatty acid dehydrogenase genes. The expression of these genes are expected to confer tolerance to sulphonylurea and glyphosate herbicides and  increased levels of monounsaturated fatty acid and decreased levels of polyunsaturated fatty acids.

PV-GMGT04, PHP19340 and PHP17752

DNA insert from GTS 40-3-2 vector PV-GMGT04
The soybean line GTS 40-3-2 was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. This genetically engineered soybean line contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows GTS 40-3-2 to survive the otherwise lethal application of glyphosate.

DNA insert from 305423 vector PHP19340 and PHP17752
The intended effect of the modification in 305423 soybean is to produce soybean seeds with increased levels of monounsaturated fatty acid and decreased levels of polyunsaturated fatty acids through the insertion of the microsomal omega-6 desaturase gene (FAD2-1) into soybean. Transcription of the gene fragment acts to silence the expression of the endogenous soybean omega-6 desaturase, which results in an increased level of oleic acid and decreased levels of linoleic and linolenic acids in the soybean seed. The acetolactate synthase (als gene) that confers tolerance to a sulfonylurea herbicide was used as a selectable marker for the transformation.

For additional information on this LMO, please refer to the records of the parental LMOs.

The DNA insert from  PHP19340 and PHP17752 contains a fragment corresponding to 40% of the soy omega-6-desaturase. Upon transcription of the fragment, the resulting transcript acts to silencing the endogenous gene by RNA interference.