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Modified Organism
MON-877Ø1-2 - Insect resistant soybean
Record information and status
Record ID
103079
Status
Published
Date of creation
2012-02-09 16:29 UTC (bchrecords@inspection.gc.ca)
Date of last update
2014-01-20 20:47 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-20 20:47 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant soybean
Transformation event
MON 87701
Unique identifier
MON-877Ø1-2
Developer(s)
Monsanto Canada Inc.
Description
The soy plant was modified with the insertion of the Cry1Ac protein which provides protection from feeding damage caused by targeted lepidopteran pests, such as primary target pests velvetbean caterpillar (Anticarcia gemmatalis), soybean looper (Pseudoplusia includens), soybean anxil borer (Epinotia aporema), and sunflower looper (Rachiplusia nu).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Characteristics of the transformation process
Vector
PV-GMIR9
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
rbcS Promoter
1.72 Kb
 
 
rbcS Transit Peptide
0.26 Kb
 
 
Cry1Ac
3.54 Kb
 
 
α' subunit of β-conglycinin gene terminator
0.44 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
MON 87701 was developed through transformation of soybean meristem tissues using the binary transformation plasmid PV-GMIR9 which contains two T-DNAs delineated by left and right border sequences which facilitate transformation. The first T-DNA, designated as T-DNA I, contains the cry1Ac expression cassette. The second T-DNA, designated as T-DNA II, contains the cp4 epsps expression cassette.

The Cry1Ac coding sequence was modified for plant optimised codons and resulted in a single amino acid change at L766S with four additional codons at the N-terminus from the CTP2 genetic element.

Molecular characterization of MON 87701 by Southern blot analyses demonstrated that the DNA inserted into the soybean genome is present at a single locus and contains one functional copy of the cry1Ac expression cassette. No TDNA II (cp4 epsps gene expression cassette) genetic elements or backbone sequences from the transformation plasmid were detected in MON 87701. In addition, no partial genetic elements, linked or unlinked to the inserted expression cassette were detected.

T-DNA II expression Cassette: FMV 35S promoter >> EPSPS Leader >> CTP2 >> EPSPS gene >> rbcS-E9 gene terminator
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
Utilizing a vector with two T-DNAs is the basis for an effective approach to generate marker-free plants. It allows for the TDNA with the traits of interest (T-DNA I) and the T-DNA encoding the selectable marker (T-DNA II) to be inserted into two independent loci within the genome of the plant. Following selection of the transformants, the inserted T-DNA encoding the selectable marker can be segregated from progeny through subsequent traditional breeding and genetic selection processes, while the inserted T-DNA containing the trait(s) of interest is maintained resulting in an LMO that marker-free and contains only the cry1Ac expression cassette.
Other relevant website address or attached documents

Records referencing this document (24)
IDDescription
24record(s) found
Country's Decision or any other Communication9 records
Modified Organism3 records
Organization4 records
Risk Assessment8 records