Potato modified for decreased sugar content | BCH-LMO-SCBD-104352 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 07 Nov 2012
Living Modified Organism identity
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Potato modified for decreased sugar content
EN
Potato with the change of sugar content in tubers
No
T-DNA section was inserted into potato plant, to express either an original or modified phosphofructokinase (PFK or PFKII respectively) gene in potato tubers driven by the tuber specific promoter of the B33 patatin gene.

Incresed PFK activity results in an increase in sugar metabolism via glycolosis and thus a decrease in the over all sugar content in potato tubers. PFKII was modified for improved expression and activity in tubers stored at lower temperatures compared to the native form of the gene. 

The gene is not expressed in above-ground parts of plants, and the viability of transgenic plants is not changed. The plants further contain  selective gene nptII (neo) expressed under in vitro conditions in all parts of plants, and inducing the resistance of a plant to the antibiotic kanamycin.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pB33PFKBin, derived from pBin19
EN
  • Agrobacterium-mediated DNA transfer
 
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-104350-1 Phosphofructokinase gene | Lactobacillus bulgaricus
    Protein coding sequence | Changes in quality and/or metabolite content (Carbohydrates)
  • BCH-GENE-SCBD-100273-4 B33 gene promotor | Solanum tuberosum (Potato, SOLTU)
    Promoter
Transformation carried out with one of two PFK gene sequences. One expressing the native protein with the Lactobacillus bulgaricus codon. The other expressing PFK with a codon sequence optimised for expression in plants and producing a protein with improved activity in tubers stored at lower temperatures.
EN
LMO characteristics
EN
  • Food
  • Research
Detection method(s)
EN
Additional Information
The field trial was authorized in the Czech Republic in April 2006, extended on request of a consent holder in 2010 and terminated in December 2011.
EN
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 1

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