| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
Potato modified for decreased sugar content
Record information and status
Record ID
104352
Status
Published
Date of creation
2012-11-07 10:57 UTC (hana_jirakova@env.cz)
Date of publication
2012-11-12 17:21 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for decreased sugar content
Transformation event
Potato with the change of sugar content in tubers
Developer(s)
Dr. Oldrich Navratil
senior scientist
Institute of Experimental Botany, AS CR (IEB)
Prague
Czech Republic
Phone:+ 420 225 106 455
Email:ueb@ueb.cas.cz
Url:Institute of Experimental Botany, AS CR
Description
T-DNA section was inserted into potato plant, to express either an original or modified phosphofructokinase (PFK or PFKII respectively) gene in potato tubers driven by the tuber specific promoter of the B33 patatin gene.

Incresed PFK activity results in an increase in sugar metabolism via glycolosis and thus a decrease in the over all sugar content in potato tubers. PFKII was modified for improved expression and activity in tubers stored at lower temperatures compared to the native form of the gene. 

The gene is not expressed in above-ground parts of plants, and the viability of transgenic plants is not changed. The plants further contain  selective gene nptII (neo) expressed under in vitro conditions in all parts of plants, and inducing the resistance of a plant to the antibiotic kanamycin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Characteristics of the transformation process
Vector
pB33PFKBin, derived from pBin19
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
B33 gene promotor
0.00 Kb
 
 
Phosphofructokinase gene
0.00 Kb
 
 
Octopine Synthase Gene Terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Transformation carried out with one of two PFK gene sequences. One expressing the native protein with the Lactobacillus bulgaricus codon. The other expressing PFK with a codon sequence optimised for expression in plants and producing a protein with improved activity in tubers stored at lower temperatures.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Research
Additional Information
Additional Information
The field trial was authorized in the Czech Republic in April 2006, extended on request of a consent holder in 2010 and terminated in December 2011.

Records referencing this document (1)
IDDescription
1record(s) found
Risk Assessment1 record