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Modified Organism
DAS-444Ø6-6 - Herbicide Tolerant Soybean
Record information and status
Record ID
105041
Status
Published
Date of creation
2013-09-18 19:50 UTC (dina.abdelhakim@cbd.int)
Date of last update
2014-01-22 19:59 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-22 19:59 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide Tolerant Soybean
Transformation event
DAS-44406-6
Unique identifier
DAS-444Ø6-6
Developer(s)
Description
DAS-44406-6 soybean was modified with the insertion of a gene encoding aryloxyalkanoate dioxygenase and phosphinothricin N-acetyltransferase to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and glufosinate respectively. Further more a gene encoding a modified version of the EPSPS gene was inserted to confer tolerance to glyphosate.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Characteristics of the transformation process
Vector
pDAB8264
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
RB7 matrix attachment region
1.17 Kb
 
 
Histone H4 gene 3' UTR
0.66 Kb
 
 
Double Mutant 5-enolpyruvylshikimate-3-phosphate synthase
1.34 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
Histone H3 Gene II intron 1
0.00 Kb
 
 
Histone H4 gene Promoter
1.43 Kb
 
 
Polyubiquitin10 gene promoter
1.32 Kb
 
 
Aryloxyalkanoate dioxygenase gene
0.88 Kb
 
 
ORF23 3' Untranslated region
0.46 Kb
 
 
CsVMV promoter
0.52 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
ORF1 3' Untranslated region
0.17 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Molecular characterization by Southern blot analyses of DAS-444Ø6-6 soybean confirmed that a single, intact DNA insert containing the aad-12, 2mepsps and pat gene expression cassettes was stably integrated into the soybean genome. Southern blot analyses also confirmed the absence of the plasmid backbone DNA in DAS-444Ø6-6 soybean. The integrity of the inserted DNA was demonstrated in five different breeding generations. Data from segregating generations confirmed the predicted Mendelian inheritance pattern. These data confirmed the stability of DAS-444Ø6-6 soybean during traditional breeding procedures.

The H4A748 promoter also includes an intron from the Histone 3 gene from Arabidopsis thaliana.
LMO characteristics
Modified traits
  • Tolerance to 2,4-Dichlorophenoxyacetic acid
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (8)
IDDescription
8record(s) found
Country's Decision or any other Communication3 records
Risk Assessment4 records
Submissions from Parties, other Governments or relevant organizations1 record