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Modified Organism
Herbicide tolerant canola
Record information and status
Record ID
110268
Status
Published
Date of creation
2016-05-05 19:36 UTC (dina.abdelhakim@cbd.int)
Date of publication
2016-05-05 19:36 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide tolerant canola
Transformation event
5715
Developer(s)
Description
A canola event that is tolerant to the sulfonylurea herbicides tribenuron-methyl and thifensulfuron-methyl.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Characteristics of the transformation process
Techniques used for the modification
  • Oligonucleotide-directed mutagenesis
Genetic elements construct
 
Acetohydroxy acid synthase 3 gene Promoter
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Acetohydroxy acid synthase 3 gene
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Acetohydroxy acid synthase 3 gene terminator
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Acetohydroxy acid synthase 1 gene promoter
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Acetohydroxy acid synthase 1 gene
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Acetohydroxy acid synthase 1 gene terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
Cibus Canada Inc. has developed a canola event that is tolerant to the sulfonylurea herbicides tribenuron-methyl and thifensulfuron-methyl. The development of canola event 5715 was accomplished by conventional breeding of two herbicide tolerant mutants, one newly produced and one commercially available. Cibus Canada Inc. utilized an oligonucleotide-directed mutagenesis approach known as the Rapid Trait Development System™ (RTDS™), which included the application of tissue culture techniques that generated plant cells more receptive to mutagenesis.

Brassica napus (B. napus) carries two complete genomes designated "A" and "C". Each genome has an acetohydroxyacid synthase (AHAS) gene (BnAHAS1 gene in the C genome and BnAHAS3 in the A genome) coding for an AHAS enzyme.

Tolerance to the sulfonylurea herbicides is achieved in canola event 5715 through the presence of the same single point mutation, known as the PM2 mutation, in both the BnAHAS1 and BnAHAS3 genes. This mutation results in modified AHAS enzymes carrying a single amino acid substitution which renders them insensitive to tribenuron-methyl and thifensulfuron-methyl.

It should be noted that the PM2 mutation confers tolerance to a range of AHAS-inhibiting herbicides commonly referred to as the "group 2" herbicides (i.e. the imidazolinones, pyrimidinylthiobenzoates, sulfonylamino-carbonyltriazolinones, sulfonylureas and triazolopyrimidines).
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents