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Living Modified Organism
(LMO)
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Cisgenic apples modified for elevated anthocyanin levels
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Multiple events
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Person:Plant Research International, Dept. Genetics and BreedingPO Box 59Wageningen,
6700 AB, Netherlands (Kingdom of the)Phone: +31 (0)317 48 36 80,Fax:Email: info@wur.nl,Website: http://www.wur.nl/uk/,Related OrganizationPlant Research International (PRI)Academic or research institutePO Box 59Wageningen,
6700 AB, Netherlands (Kingdom of the)Phone: +31 (0)317 48 36 80,Fax:Email: info@wur.nl,Website: http://www.wur.nl/uk/,
Apple cultivars Jumani and Gala were modified with the insertion of the MdMYB10(R6) gene form Malus x domestica cultivar 'red field' resulting in an increased expression of anthocyanin leading to red fleshed apples.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12099-5 Organism Malus domestica (Apple, MALDO)Crops
Cultivars: Jumani and Gala
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pMinMYB
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-110605-2 MdMYB10 transcription factor | Malus domestica (Apple, MALDO)Protein coding sequence | Changes in quality and/or metabolite content (Flavonoids (e.g. anthocyanin))
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BCH-GENE-SCBD-110606-1 Stopper SequenceTerminator
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BCH-GENE-SCBD-110607-1 MdMYB10 transcription factor R6 promoter | Malus domestica (Apple, MALDO)Promoter
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BCH-GENE-SCBD-110608-1 MdMYB10 transcription factor terminator | Malus domestica (Apple, MALDO)Terminator
A naturally occurring mutant of the MdMYB10 gene was identified in the cv. ‘Red Field’, in which the promoter carries a six-fold repeat of a 23 bp DNA binding domain (R6). The wild-type form only has one copy of this domain (R1). This mutation has been found in nearly all naturally occurring red-fleshed apples.
In apple, red-fleshed varieties are not considered to be very tasteful. In order to combine elevated anthocyanin levels with potential health-promoting antioxidant effect with high-quality of taste and other characteristics the R6 form of MdMYB10 was introduced into elite varieties.
Due to the long generation time and self-incompatibility of apple this cannot be achieved by classical breeding. The gene including its R6 promoter and its own terminator was the only genetic element present on the region to be inserted. On either side of the gene within the T-DNA a 11 bp stopper sequence was placed to avoid undesired readthrough.
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In apple, red-fleshed varieties are not considered to be very tasteful. In order to combine elevated anthocyanin levels with potential health-promoting antioxidant effect with high-quality of taste and other characteristics the R6 form of MdMYB10 was introduced into elite varieties.
Due to the long generation time and self-incompatibility of apple this cannot be achieved by classical breeding. The gene including its R6 promoter and its own terminator was the only genetic element present on the region to be inserted. On either side of the gene within the T-DNA a 11 bp stopper sequence was placed to avoid undesired readthrough.
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- Research
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