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Modified Organism
MON-ØØ81Ø-6 - YieldGard™ maize
Record information and status
Record ID
14750
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-05-24 18:43 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-05-24 18:43 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
YieldGard™ maize
Transformation event
MON810
Unique identifier
MON-ØØ81Ø-6
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Insect-resistant maize produced by inserting the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki HD-1. The genetic modification affords resistance to attack by the European corn borer (ECB), Ostrinia nubilalis.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Related LMOs
PH-MON8Ø9-2 - Insect-resistant maize MON809
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glyphosate
Insect Resistant Maize MON801
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glyphosate
Characteristics of the transformation process
Vector
PV-ZMBK07 and PV-ZMGT10
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Cry1Ab
3.46 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The transgenic maize line MON810 was genetically engineered to resist ECB by producing its own insecticide. This line was developed by introducing a synthetic version of the cry1Ab gene, isolated from the soil bacterium Bacillus thuringiensis (Bt) which was modified to enhance the expression of the Cry1Ab protein in plants, however the resulting amino acid sequence is identical to the native protein.

Molecular studies demonstrated that a single truncated copy of the cryIAb coding sequence was integrated into the corn genome along with the enhanced cauliflower mosaic virus 35S promoter (P-e35S), and the hsp 70 intron (I-Hsp70).  The nos terminator was not integrated into MON810 due to a truncation of the 3' end of the gene cassette.  Western analysis confirmed that a truncated Cry1Ab protein of approximately 91 kD (native Cry1Ab had a molecular weight of approximately 131 kD) was inserted into the genome. 

Corn event MON 810 was produced by microprojectile bombardment of embryogenic corn tissue with plasmids PVZMBK07 and PV-ZMGT10. However plasmid vector PV-ZMGT10 was not integrated into the plant genome. Further Southern blot analysis indicated that the genes for glyphosate tolerance (CP4 EPSPS) and antibiotic resistance (neo) were not transferred to line MON 810 and the absence of the CP4 EPSPS and gox gene products was also confirmed by Western blotting. The CP4 EPSPS and GOX protein encoding genes were presumed to have been inserted into the initial transformant at a separate genetic loci from the cry1Ab gene and then subsequently lost through segregation during the crossing events leading to line MON810.

Southern analysis confirms that the nptII gene (originally present in PVZMBK07 and PV-ZMGT10) is not present in MON 810.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel

Records referencing this document (136)
IDDescription
136record(s) found
Country's Decision or any other Communication40 records
Information Resource4 records
Modified Organism27 records
Organization34 records
Risk Assessment31 records