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NaturGard KnockOut™ maize
This LMO contains two copies of a truncated synthetic version of
the full length cry1Ab gene from Bacillus
thuringiensis subsp. kurstaki. The synthetic
truncated cry1Ab gene encodes a protein that corresponds
to the first 648 amino acids of the N-terminal of the 1155 amino
acid full length native Cry1Ab protein and includes the portion of
the native protein that is necessary for insect control.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Proprietary Ciba Seeds inbred maize line CG00526
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Phosphinothricin N-acetyltransferase gene - Streptomyces hygroscopicus - STRHY
Resistance to herbicides - Glufosinate
Cry1Ab - Bacillus thuringiensis - Bt, Bacillus, BACTU
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Beta-lactamase gene - Escherichia coli - ECOLX
Resistance to antibiotics - Ampicillin
Phosphoenolpyruvate Carboxylase gene promoter - Zea mays - Maize, Corn, MAIZE
Calcium-dependent protein kinase promoter - Zea mays - Maize, Corn, MAIZE
Phosphoenolpyruvate carboxylase, intron 9 - Zea mays - Maize, Corn, MAIZE
Additional information concerning the cry1Ab gene
inserts in this LMO:
The expression of the two copies of the cry1Ab genes are under the
control either of a pollen-specific promoter the from a
calcium-dependent protein kinase or green tissue-specific promoter
phosphoenolpyruvate carboxylase. Both promoters were isolated from
maize. The termination sequences for both of genes was from
cauliflower mosaic virus (CaMV), a known plant pest.
Additional information concerning the bar gene
insert in this LMO:
This LMO contains one copy of the bar gene from
Streptomyces hygroscopicus which encodes for
phosphinotricin acetyltransferase (PAT) that confers resistance to
glufosinate herbicide. The bar gene is under the
regulation of the 35S promoter and the 35S terminator from the
cauliflower mosaic virus (CaMV).
Additional information concerning the bla gene
insert in this LMO:
The bla gene from Escherichia coli is not
expressed in plant cells, but was employed as a selectable trait
for screening bacterial colonies for the presence of the plasmid
Additional information on the inserted genetic
Two plasmids, pCIB3064 and pCIB4431 were used as vectors for the
transformation of Bt176 maize. Both are derivatives of the plasmid
PUC18, which has a molecular weight of 2.7 kb and contains
sequences such as prokaryotic gene bla and gene lacZ.
Plasmid pCIB3064 contains one copy of the bar gene which
is under the regulation of the 35S promoter and the 35S terminator
from the cauliflower mosaic virus (CaMV). The plasmid
pCIB4431 contains two copies of a synthetic truncated cry1Ab gene;
the first copy which is under the regulation of a promoter derived
from maize phosphoenolpyruvate carboxylase gene and the CaMV 35S
terminator; and the second copy which is under the regulation of a
promoter derived from maize calcium-dependent protein kinase gene
("pollen promoter') and the CaMV 35S terminator.
There are uncertainties regarding the copy number of the inserts in
Bt176. Evidence suggests that 2-5 copies of the inserts may be
present (see document below on the molecular characterization of
- Resistance to antibiotics
- Resistance to diseases and pests
- Lepidoptera (butterflies and moths)
- European corn borer (Ostrinia nubilalis)
- Resistance to herbicides