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Modified Organism
ACS-BNØØ3-6 - InVigor™ canola
Record information and status
Record ID
14755
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-05-30 14:24 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-05-30 14:24 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
InVigor™ canola
Transformation event
RF3
Unique identifier
ACS-BNØØ3-6
Developer(s)
Description
Canola fertility restoration system displaying glufosinate herbicide tolerance. Contains the barstar gene from Bacillus amyloliquefaciens, and the bar gene encoding phosphinothricin N-acetyltransferase (PAT) from Streptomyces hygroscopicus to confer tolerance to the herbicide phosphinothricin (Glufosinate ammonium).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Related LMOs
ACS-BNØØ5-8 - InVigor™ canola
Changes in physiology and/or production - Reproduction - Male sterility Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
pTHW118
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
pTA29 pollen specific promoter
1.51 Kb
 
 
Barstar
0.27 Kb
 
 
Barstar gene terminator
0.04 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
rbcS Promoter
1.73 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Transcript 7 gene 3' untranslated region
0.21 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
On the N-terminal  two codons of the wild type bar coding region have been substituted for the codons ATG and GAC.

Southern blot and PCR analysis indicated that the LMO contains one complete copy of the transformation cassette arranged in an inverted repeat orientation to a second partial copy (comprising of a portion of the TA29 promoter, the barstar coding sequence, the nos terminator sequence and a non-initiation codon of the bar gene)  of the transformation cassette adjacent to the left T-DNA boundary.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The transgenic line RF3 was produced by genetically engineering plants to restore fertility in the hybrid line. Transgenic RF3 plants contain the barstar gene, isolated from Bacillus amyloliquefaciens. The barstar gene codes for a ribonuclease inhibitor (barstar enzyme) expressed only in the tapetum cells of the pollen sac during anther development. The ribonuclease inhibitor (barstar enzyme) specifically inhibits barnase RNAse expressed by male sterile lines. Together, the RNAse and the ribonuclease inhibitor form a very stable one-to-one complex, in which the RNAse is inactivated. As a result, when pollen from the restorer line RF3 is crossed to a male sterile line, the resultant progeny express the RNAse inhibitor in the tapetum cells of the anthers, allowing hybrid plants to develop normal anthers and restoring fertility.

RF3 was also engineered to express tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®). Glufosinate chemically resembles the amino acid glutamate and acts to inhibit an enzyme, called glutamine synthetase, which is involved in the synthesis of glutamine. Essentially, glufosinate acts enough like glutamate, the molecule used by glutamine synthetase to make glutamine, that it blocks the enzyme's usual activity. Glutamine synthetase is also involved in ammonia detoxification. The action of glufosinate results in reduced glutamine levels and a corresponding increase in concentrations of ammonia in plant tissues, leading to cell membrane disruption and cessation of photosynthesis resulting in plant withering and death. 

Glufosinate tolerance in this line was the result of introducing a gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces hygroscopicus. The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound.
Other relevant website address or attached documents

Records referencing this document (38)
IDDescription
38record(s) found
Country's Decision or any other Communication13 records
Modified Organism6 records
Organization12 records
Risk Assessment7 records