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Modified Organism
ACS-BNØØ4-7 - InVigor™ canola
Record information and status
Record ID
14756
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-05-27 19:19 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-05-27 19:19 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
InVigor™ canola
Transformation event
MS1 (B91-4)
Unique identifier
ACS-BNØØ4-7
Developer(s)
Description
Canola male-sterility system displaying glufosinate herbicide tolerance. Contains the barnase gene from Bacillus amyloliquefaciens and the bar gene encoding phosphinothricin N-acetyltransferase from Streptomyces hygroscopicus to confer tolerance to the herbicide phosphinothricin (Glufosinate ammonium).  Also contains the neomycin phosphotransferase II (npt II) gene conferring resistance to the antibiotic kanamycin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Related LMOs
ACS-BNØØ1-4 - InVigor™ canola
Changes in physiology and/or production - Fertility restoration Resistance to antibiotics - Kanamycin Resistance to herbicides - Glufosinate
Show detection method(s)
ACS-BNØØ2-5 - InVigor™ canola
Changes in physiology and/or production - Fertility restoration Resistance to antibiotics - Kanamycin Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
pTTM8RE
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
pTA29 pollen specific promoter
1.50 Kb
 
 
Barnase
0.34 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
rbcS Promoter
1.84 Kb
 
 
rbcS Transit Peptide
0.16 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.50 Kb
 
 
Transcript 7 gene 3' untranslated region
0.20 Kb
 
 
Nopaline Synthase Gene Promoter
0.40 Kb
 
 
Neomycin Phosphotransferase II
1.00 Kb
 
 
Octopine Synthase Gene Terminator
0.90 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Southern blot analysis indicated that a single copy of the T-DNA was inserted into the host genome at a single site. There was no indication from the tests that any sequences from the vector backbone were integrated into the genome.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The transgenic MS1 plants do not produce viable pollen grains and cannot self-pollinate. In order to restore fertility in the hybrid progeny, line MS1 must be pollinated by a modified plant containing a fertility restorer gene. The resultant hybrid seed derived from the cross generates hybrid plants that produce pollen and are completely fertile.

The male-sterile trait was introduced in MS1 by inserting the barnase gene, isolated from Bacillus amyloliquefaciens, a common soil bacterium that is frequently used as a source for industrial enzymes. The barnase gene encodes for a ribonuclease enzyme (RNAse) that is expressed only in the tapetum cells of the pollen sac during anther development. The RNAse affects RNA production, disrupting normal cell functioning and arresting early anther development, thus leading to male sterility.

MS1 was also engineered to express tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®). Glufosinate chemically resembles the amino acid glutamate and acts to inhibit an enzyme, called glutamine synthetase, which is involved in the synthesis of glutamine. Essentially, glufosinate acts enough like glutamate, the molecule used by glutamine synthetase to make glutamine, that it blocks the enzyme's usual activity. Glutamine synthetase is also involved in ammonia detoxification. The action of glufosinate results in reduced glutamine levels and a corresponding increase in concentrations of ammonia in plant tissues, leading to cell membrane disruption and cessation of photosynthesis resulting in plant withering and death.

Records referencing this document (22)
IDDescription
22record(s) found
Country's Decision or any other Communication11 records
Modified Organism5 records
Organization3 records
Risk Assessment3 records