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Modified Organism
MON-8Ø2ØØ-7 - YieldGard™ maize
Record information and status
Record ID
14786
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-09-14 18:33 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-14 18:33 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
YieldGard™ maize
Transformation event
MON802
Unique identifier
MON-8Ø2ØØ-7
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The transgenic maize line MON802 was genetically engineered to resist ECB by producing its own insecticide. This line was developed by introducing the cry1Ab gene, isolated from the common soil bacterium Bacillus thuringiensis (Bt), into the maize line by particle acceleration (biolistic) transformation. MON802 was further engineered to express resistance to glyphosate, the active ingredient in the herbicide Roundup®, allowing for its use as a weed control option. In order to obtain field tolerance to glyphosate herbicide, two novel genes, CP4 epsps and goxv247, were introduced maize by particle acceleration (biolistic) transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
PV-ZMBK15 and PV-ZMGT03
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.64 Kb
 
 
Hsp70 intron
0.81 Kb
 
 
Cry1Ab
3.47 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.64 Kb
 
 
Hsp70 intron
0.81 Kb
 
 
Chloroplast transit peptide 2
0.31 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
 
CaMV Enhanced 35S promoter
0.64 Kb
 
 
Hsp70 intron
0.81 Kb
 
 
rbcS Transit Peptide
0.26 Kb
 
 
Glyphosate oxidoreductase gene
1.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Plasmid PV-ZMBK15 contained the synthetic cry1Ab gene and the CP4 EPSPS encoding gene from A. tumefaciens strain CP4. Plasmid PV-ZMGT03 the goxv247 gene from Ochrobactrum anthropi.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The cry1Ab gene produces the insect control protein Cry1Ab, a delta-endotoxin. The Cry1Ab protein produced by the Bt maize is identical to that found in nature and in commercial Bt spray formulations. Cry proteins, of which Cry1Ab is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. Cry1Ab is lethal only when eaten by the larvae of lepidopteran insects (moths and butterflies), and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for the delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.

In plants, the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (abbreviated EPSPS) plays a key role in the biochemical pathway that results in the synthesis of the aromatic amino acids phenylalanine, tyrosine, and tryptophan. This enzyme is only present in plants and microorganisms, such as bacteria and fungi, and is not present in animals and humans. The simple amino acid analogue glyphosate selectively inhibits the activity of the EPSPS enzyme, thus shutting off aromatic amino acid synthesis. Because these amino acids are needed for protein synthesis, which is required for plant growth and maintenance, the application of glyphosate quickly results in plant death. EPSPS is not present in mammals, birds or aquatic life forms, which do not synthesize their own aromatic amino acids. For this reason, glyphosate has little toxicity to these organisms.

A gene encoding a glyphosate-tolerant form of the EPSPS enzyme was isolated from the CP4 strain of Agrobacterium tumefaciens, a common soil bacterium, and introduced into the maize genome using micro-particle bombardment. MON802 contains a third gene that codes for a modified version of the enzyme glyphosate oxidase (GOX), which accelerates the normal breakdown of glyphosate into two non-toxic products, aminomethylphosphonic acid (AMPA) and glyoxylate. AMPA is the principal breakdown product of glyphosate and is degraded by several microorganisms, while glyoxylate is commonly found in plant cells and is broken down by the glyoxylic pathway for lipid metabolism. The GOX encoding gene (goxv247) was isolated from the bacterium Ochrobactrum anthropi strain LBAA.
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record