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Modified Organism
MON-ØØØ73-7 - Roundup Ready™ canola
Record information and status
Record ID
14795
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-01-22 21:18 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-22 21:18 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Roundup Ready™ canola
Transformation event
RT73 (GT73)
Unique identifier
MON-ØØØ73-7
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Glyphosate herbicide tolerant canola produced by inserting the epsps gene encoding the enzyme 5-enolypyruvylshikimate-3-phosphate synthase (EPSPS) and glyphosate oxidase (gox) from Ochrobactrum anthropi. These modifications confer tolerance to the herbicide glyphosate.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Point of collection or acquisition of the recipient organism
Cultivar: Westar (Standard variety used in the Western Canadian Cooperative Rapeseed Test (Co-Op Test))
Related LMOs
MON-89249-2 - Roundup Ready™ canola
Resistance to herbicides - Glyphosate
Characteristics of the transformation process
Vector
PV-BNGT04
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S promoter
0.00 Kb
 
 
Chloroplast transit peptide 2
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5-enolpyruvylshikimate-3-phosphate synthase gene
0.00 Kb
 
 
rbcS-E9 gene terminator
0.00 Kb
 
 
FMV 35S promoter
0.00 Kb
 
 
rbcS Transit Peptide
0.00 Kb
 
 
Glyphosate oxidoreductase gene
0.00 Kb
 
 
rbcS-E9 gene terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The Glyphosate oxidoreductase varies from the wild type version of the gene at 3 amino acid sites (G85S, R153K and R334H) and is designated as goxv247.

The EPSPS gene was codon optimised for plant expression.

PCR and southern blot analysis indicated that MON-ØØØ73-7 contains a single insertion event containing one copy of the T-DNA from plasmid PV-BNGT04. No genetic elements from outside of the right and left borders of the plasmid were transferred into or are present in the genomic DNA of the LMO.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Additional Information
The epsps gene codes for the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that is present in all plants, bacteria and fungi. The epsps gene put into GT73 was isolated from strain CP4 of the common soil bacterium Agrobacterium tumefaciens and is a glyphosate tolerant form of EPSPS. The EPSPS enzyme is part of an important biochemical pathway in plants called the shikimate pathway, which is involved in the production of aromatic amino acids and other aromatic compounds. When conventional canola plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is not present in mammals, birds or aquatic life forms, which do not synthesize their own aromatic amino acids. For this reason, glyphosate has little toxicity to these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.

The canola line GT73 contains a second gene that codes for a modified version of glyphosate oxidase (GOX) enzyme that is ubiquitous in nature. The goxv247 gene inserted into GT73 was isolated from strain LBAA of the bacterium Ochrobactrum anthropi. Glyphosate oxidase (GOX) enzyme accelerates the normal breakdown of the herbicide glyphosate into two non-toxic compounds, aminomethylphosphonic acid (AMPA) and glyoxylate. AMPA is the principal breakdown product of glyphosate and is degraded by several microorganisms, while glyoxylate is commonly found in plant cells and is broken down by the glyoxylic pathway for lipid metabolism.
Other relevant website address or attached documents

Records referencing this document (50)
IDDescription
50record(s) found
Country's Decision or any other Communication18 records
Modified Organism3 records
Organization15 records
Risk Assessment14 records