| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
MON-ØØØ21-9 x MON-ØØ81Ø-6 - Roundup Ready™ YieldGard™ maize
Record information and status
Record ID
14892
Status
Published
Date of creation
2006-06-05 14:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-07-24 13:45 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 13:45 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Roundup Ready™ YieldGard™ maize
Transformation event
GA21 x MON810
Unique identifier
MON-ØØØ21-9 x MON-ØØ81Ø-6
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
This LMO is a stacked insect-resistant and glyphosate-tolerant maize derived from conventional cross-breeding of MON-ØØØ21-9 and MON-ØØ81Ø-6. 
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
MON-ØØ81Ø-6 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
MON-ØØØ21-9 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
pDPG434, PV-ZMBK07 and PV-ZMGT10
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Cry1Ab
3.46 Kb
 
 
Rice actin 1 gene promoter
1.37 Kb
 
 
Rice actin 1, intron
0.00 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
transcribed element
 
 
5-enolpyruvylshikimate-3-phosphate synthase
1.34 Kb
 
 
Nopaline Synthase Gene Terminator
0.24 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from GA21, vector pDPG434:

The GA21 line of maize was genetically engineered, by particle acceleration (biolistic) transformation, to be tolerant of glyphosate-containing herbicides.

DNA insert from MON810, vectors PV-ZMBK07 and PV-ZMGT10:

MON810 contains a truncated portion of a synthetic form of the cry1Ab gene. Two constructs PV-ZMBK07 and PV-ZMGT10 have been used for transformation, but molecular analyses showed that MON810 does not contain any element from PV-ZMGT10 construct and only the elements from construct PV-ZMBK07 have been integrated into its genome.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel
Detection method(s)
Additional information
GA21 contains a single single insertion site with 3 complete copies of mEPSPS cassette plus 3 incomplete copies as follows: (1) a mEPSPS gene cassette, truncated at the 5' end of the rice actin promoter sequence; (2) three complete internal mEPSPS gene cassettes; (3) a partial mEPSPS gene cassette containing the promoter, intron, otp, and a partial mEPSPS coding sequence terminating in a stop codon; and (4) an additional partial gene cassette at the 3' end containing only the rice actin promoter and 5' mRNA leader sequence, but truncating before the start of the rice actin intron, followed by corn genomic DNA.

MON810 contains one integrated DNA consisting of P-e35S, I-Hsp70 and cry1Ab. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.
Additional Information
Other relevant website address or attached documents

Records referencing this document (11)
IDDescription
11record(s) found
Country's Decision or any other Communication4 records
Organization4 records
Risk Assessment3 records