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Modified Organism
NMK-89576-1 - Superior NewLeaf™ potato
Record information and status
Record ID
14900
Status
Published
Date of creation
2006-06-05 14:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-04-26 15:39 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-04-26 15:39 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Superior NewLeaf™ potato
Transformation event
SPBT02-5
Unique identifier
NMK-89576-1
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The transgenic potatoes were genetically engineered to be resistant to attack by Colorado potato beetle  by producing their own insecticide. These lines were developed by introducing the cry3A gene.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar: Superior
Related LMOs
NMK-89724-5 - Superior NewLeaf™ potato
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
Characteristics of the transformation process
Vector
PV-STBT02
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Cry3A
1.80 Kb
 
 
rbcS-E9 gene terminator
0.36 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The coding sequence of the Cry3A gene was modified to plant preferred codons. This resulted in changes to 399 of 1791 nucleotides but there were no changes to the resulting amino acid sequence.

Southern blot analysis indicated that they Cry3A gene and regulatory elements were incorporated into the host genome. The transformation cassette also contained an nptII gene however this was not transferred into the genome. Additionally segments of the ori-v and ori322 coding sequences, which ere outside the left and right borders, were detected.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The Cry3A protein expressed in these transgenic potato cultivars is identical to that found in nature and in commercial Bt spray formulations. Cry proteins, of which Cry3A is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow causing gut paralysis and eventual death due to bacterial sepsis. Cry3A is insecticidal only when eaten by the larvae of coleopteran insects such as Colorado potato beetle and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.
Other relevant website address or attached documents

Records referencing this document (8)
IDDescription
8record(s) found
Country's Decision or any other Communication5 records
Modified Organism1 record
Risk Assessment2 records