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Modified Organism
NMK-89653-6 - New Leaf™ Y Russet Burbank potato
Record information and status
Record ID
14903
Status
Published
Date of creation
2006-06-05 14:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-04-29 20:42 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-04-29 20:42 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
New Leaf™ Y Russet Burbank potato
Transformation event
RBMT15-101
Unique identifier
NMK-89653-6
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Potatoes with insect-resistance and resistance to potato virus Y through inclusion of the cry3A gene from Bacillus thuringiensis which confers resistance to coleopteran pests, and DNA sequences corresponding to potato virus Y (PVY) coat protein domains which confers resistance to PVY. 
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar: Russet Burbank
Related LMOs
NMK-8993Ø-4 - Shepody NewLeaf™ Y potato
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Coleoptera (beetles), Viruses - Potato virus Y (PVY)
NMK-89935-9 - Shepody NewLeaf™ Y potato
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Coleoptera (beetles), Viruses - Potato virus Y (PVY)
Characteristics of the transformation process
Vector
PV-STMT15
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
rbcS Promoter
1.70 Kb
 
 
Cry3A
1.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
Nopaline Synthase Gene Promoter
0.30 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.45 Kb
 
 
FMV 35S promoter
0.57 Kb
 
 
HSP17.9 Leader Sequence
0.08 Kb
 
 
PVY coat protein
0.81 Kb
 
 
rbcS-E9 gene terminator
0.63 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Integration of the T-DNA occurred at three to four loci. At least one locus contained two copies of the T-DNA organized in inverted orientations. For two copies of the T-DNA, transfer was incomplete at the right border resulting in an incomplete copy of the figwort mosaic virus (FMV) 35S promoter associated with the PVY CP gene.

One of the cry3Aa genes also lacked the Arabidopsis small subunit promoter and a portion of the 5' end of the gene. The NOS terminator region of this gene cassette was intact. One of the T-DNAs also had an incomplete NOS promoter region associated with an intact NPTII coding region.

The coding regions of all the other genetic elements were intact. The analyses also showed that no plasmid sequences beyond the left and right borders were transferred.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Additional Information
The transgenic potato line RBMT15-101 was produced using recombinant DNA techniques and contain two novel genes, whose individual expression results in resistance to attack by Colorado potato beetle (CPB; Leptinotarsa decemlineata) and resistance to infection by Potato Virus Y strain O (PVY-O). Resistance to attack by CPB was accomplished by introducing the cry3A gene from Bacillus thuringiensis subsp. tenebrionis, which encodes an insecticidal crystalline Cry3A delta-endotoxin protein. The insecticidal activity of Cry3A protein is due to its selective binding to specific sites localized on the brush border midgut epithelium of susceptible insect species. Following binding, cation-specific pores are formed that disrupt midgut ion flow and thereby cause gut paralysis, ultimately leading to bacterial sepsis and death. Delta-endotoxins, such as the Cry3A protein expressed in CPB resistant potato lines, exhibit highly selective insecticidal activity against a narrow range of coleopteran insects such as CPB, elm leaf beetle and yellow mealworm. Their specificity of action is directly attributable to the presence of specific receptors in the target insects. There are no receptors for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.

Pathogen-derived resistance to PVY was conferred by introducing the coat protein (CP) gene from PVY-O. The coat protein forms a protective coat around the RNA genome of the virus and comprises 95% by mass of the virus particle. Although the exact mechanism is not fully understood, these transgenic potato lines exhibit resistance to infection and subsequent disease caused by PVY through a process that is related to viral cross-protection.
Other relevant website address or attached documents

Records referencing this document (16)
IDDescription
16record(s) found
Country's Decision or any other Communication7 records
Modified Organism2 records
Risk Assessment7 records