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Modified Organism
DAS-21Ø23-5 - Insect-resistant cotton
Record information and status
Record ID
14938
Status
Published
Date of creation
2006-06-07 20:17 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-04-10 19:04 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-04-10 19:04 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect-resistant cotton
Transformation event
3006-210-23
Unique identifier
DAS-21Ø23-5
Developer(s)
Description
The cotton line 3006-210-23 was genetically engineered to resist attack from lepidopteran insect pests such as the cotton bollworm, pink bollworm, tobacco budworm, beet armyworm, and soybean looper. This insect resistance is conferred by the cry1Ac gene, originally isolated from the common soil bacterium Bacillus thuringiensis (Bt) var. kurstaki.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
Point of collection or acquisition of the recipient organism
Line 'Germain's Acala GC510'
Characteristics of the transformation process
Vector
pMYC3006
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
4ocs∆Mas2’ promoter
0.61 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
ORF25 PolyA Terminator sequence
0.73 Kb
 
 
Cry1Ac
3.47 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Coding sequences of Cry1A(c) and pat genes altered for optimal expression in plant cells.

Southern blot analysis demonstrated that event 3006-210-23 contains a single integration of the transgenic DNA, including one intact copy of the cry1Ac gene, one intact copy of the pat gene, and single, intact copies of each of the regulatory elements. Analysis also indicated that there was no integration of segments from the erythromycin resistance gene.
LMO characteristics
Modified traits
Common use(s)
  • Fiber / Textile
Additional Information
Additional Information
The cry1Ac gene produces the insect control protein Cry1Ac, a delta-endotoxin, in the plant tissues. Cry proteins, of which Cry1Ac is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. Cry1Ac is insecticidal only when eaten by the larvae of lepidopteran insects (moths and butterflies), and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.
Other relevant website address or attached documents

Records referencing this document (9)
IDDescription
9record(s) found
Country's Decision or any other Communication2 records
Modified Organism3 records
Organization2 records
Risk Assessment2 records