| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
DAS-24236-5 - Insect-resistant cotton
Record information and status
Record ID
14940
Status
Published
Date of creation
2006-06-07 20:23 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-04-08 20:00 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-04-08 20:00 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect-resistant cotton
Transformation event
281-24-236
Unique identifier
DAS-24236-5
Developer(s)
Description
The cotton line 281-24-236 was genetically engineered to resist attack from Lepidopteran insect pests such as the tobacco budworm, cotton bollworm, beet armyworm, and soybean looper. This insect resistance is conferred by the cry1F gene, originally isolated from the common soil bacterium Bacillus thuringiensis (Bt) var. aizawai.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
Point of collection or acquisition of the recipient organism
Line: 'Germain's Acala GC510'
Characteristics of the transformation process
Vector
pAGM281
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ubiquitin gene promoter
0.61 Kb
 
 
Ubiquitin Intron 1
1.99 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
ORF25 PolyA Terminator sequence
0.73 Kb
 
 
Cry1F
3.45 Kb
 
 
4ocs∆Mas2’ promoter
0.61 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Lepidopteran-resistant cotton, with insect resistance conferred by the cry1F gene from the common soil bacterium Bacillus thuringiensis (Bt) var. aizawai.

Coding sequences of Cry1F and pat genes altered for optimal expression in plant cells. The Cry1F protoxin is a chimeric, full-length δ-endotoxin comprised of the core toxin of Cry1F from Bacillus thuringiensis var. aizawai strain PS81I and nontoxic portions of Cry1Ca3 and Cry1Ab1 proteins. Together, the portions of Cry1Ca3 and Cry1Ab1 that comprise the chimeric C-terminal domain are approximately those removed by alkaline proteases during the formation of the active Cry1F core toxin.
LMO characteristics
Modified traits
Common use(s)
  • Fiber / Textile
Additional Information
Additional Information
The cry1F gene produces the insect control protein Cry1F, a delta-endotoxin, in the plant tissues. Cry proteins, of which Cry1F is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. Cry1F is insecticidal only when eaten by the larvae of lepidopteran insects (moths and butterflies), and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.
Other relevant website address or attached documents

Records referencing this document (12)
IDDescription
12record(s) found
Country's Decision or any other Communication3 records
Information Resource1 record
Modified Organism3 records
Organization2 records
Risk Assessment3 records