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Modified Organism
REN-ØØØ38-3 - Mavera™ maize
Record information and status
Record ID
15103
Status
Published
Date of creation
2006-06-19 10:17 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-01-20 21:11 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-20 21:11 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Mavera™ maize
Transformation event
LY038
Unique identifier
REN-ØØØ38-3
Developer(s)
Renessen LLC Netherlands
Url:Renessen Homepage
Description
The maize was transformed through microparticle bombardment to contain the cordapA gene from the bacterium Corynebacterium glutamicum.  The cordapA gene produces dihydrodipicolinate synthase which results in the production of grain with higher lysine content and is intended to have improved nutritional value for use as a feed.

The selectable marker gene nptII was used during the initial transformation and tissue culture process, and was removed using the Cre/Lox system following selection of the line.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
PV-ZMPQ76
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Globulin 1 Promotor
1.39 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Dihydrodipicolinate synthase chloroplast targeting sequence
0.17 Kb
 
 
Dihydrodipicolinate synthase
0.90 Kb
 
 
Globulin 1 Terminator
0.99 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Maize with increased production of the amino acid lycine through introduction of the cordapA gene from Corynebacterium glutamicum and regulated by a promoter from globulin 1 (Glb1) gene from Zea mays, rice actin gene intron (rAct1) and Z. mays chloroplast transit peptide sequence for DHDPS and a Glb1 gene 3' terminator from a non-translated region from Z. mays. The DNA used in the microprojectile bombardment also comprised a nptII cassette as a selectable marker which was flanked by loxP recombination sites. The progeny were screened for plants that did not contain the nptII gene cassette, but contained the cordapA gene cassette. The Cre recombinase protein gene, which was also integrated into the maize genome, was segregated away from the cordapA gene through subsequent breeding.

Information on the inserted DNA sequences:
The linear DNA fragment used in the transformation included two expression cassettes, each with a single copy of a gene: cordapA, and  nptII. The 3′ nontranslated region of the globulin 1 gene contains the polyadenylation signal.The nptII gene cassette confers the paromomycin resistance that permits the selection of cells containing the expression cassette. 

Vector information
The plant expression plasmid vector, PV-ZMPQ76, contains three expression cassettes, the cordapA sequence encoding a lysine-insensitive cDHDPS protein, nptII, a selectable marker flanked by loxP recombination sites and amp, a selectable marker encoding ampicillin resistance which, along with nptII was used for selection in E.coli. The 5.9 Kb fragment of this plasmid used for transformation contained only the cordapA and nptII cassettes and in the final product only the cordapA cassette was present. This was accomplished by crossing plants positive for cordapA with a maize line engineered to express the Cre recombinase protein.  The Cre recombinase in the resulting hybrid initiated the excision of the DNA fragment containing the nptII cassette at the loxP sites.

The nptII genetic element was constructed as follows: loxp >> CaMV 35S promoter >> nptII coding sequence >> ble >> NOS terminator >> loxp
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (17)
IDDescription
17record(s) found
Country's Decision or any other Communication7 records
Modified Organism1 record
Organization3 records
Risk Assessment6 records