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Modified Organism
MON-88Ø17-3 - YieldGard™ VT™ Rootworm/RR2™ Maize
Record information and status
Record ID
15106
Status
Published
Date of creation
2006-06-19 11:07 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-05-16 18:48 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-05-16 18:48 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
YieldGard™ VT™ Rootworm/RR2™ Maize
Transformation event
MON88017
Unique identifier
MON-88Ø17-3
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Corn rootworm-resistant maize line MON88017 was produced using recombinant-DNA techniques to express the cry3Bb1 gene encoding a Coleopteran-specific insecticidal protein from Bacillus thuringiensis (subsp. kumamotoensis) in order to control infestation with corn root worm, and the cp4 epsps gene from the soil bacterium Agrobacterium ssp. strain CP4.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
PV-ZMIR39
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Rice actin 1 gene promoter
0.93 Kb
 
 
Rice actin 1, intron
0.46 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.07 Kb
 
 
Rice actin 1, intron
0.46 Kb
 
 
Cry3Bb1
1.96 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.23 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The wild-type cry3Bb1 coding sequence was modified to encode six specific amino acid substitutions, resulting in the synthetic cry3Bb1 coding sequence present in the vector. The differences at the six positions are: 2A (insertion), H232R, S312L, N314T, E318K, Q349R.

Molecular analyses of MON 88017 confirmed that single copies of the cp4 epsps and cry3Bb1 genes are integrated at a single locus in the corn genome with all expression elements intact and no plasmid bacterial backbone present. Plasmid PV-ZMIR39 contains the left and right transfer DNA (T-DNA) border sequences that facilitate transformation.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The cry3Bb1 gene encodes the insect control protein Cry3Bb1, a delta-endotoxin. The toxin protects the plant from the Coleopteran insects western corn rootworm (Diabrotica virgifera), northern corn rootworm (D. barberi), and Mexican corn rootworm (D. virgifera zeae).  Cry proteins, of which Cry3Bb1 is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. Cry3Bb1 is lethal only when eaten by Coleopteran species, including corn root worm, and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for the delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.

The cp4 epsps gene encodes for a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that is highly tolerant to inhibition by glyphosate.
Other relevant website address or attached documents

Records referencing this document (86)
IDDescription
86record(s) found
Country's Decision or any other Communication27 records
Information Resource1 record
Modified Organism12 records
Organization19 records
Risk Assessment27 records