DAS-24236-5 × DAS-21Ø23-5 - WideStrike™ cotton | BCH-LMO-SCBD-15107 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
published: 19 Jun 2006 last updated: 24 Jul 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
WideStrike™ cotton
EN
281-24-236 x 3006-210-23 (MXB-13)
Yes
DAS-24236-5 × DAS-21Ø23-5
A stacked lepidopteran insect-resistant cotton derived from conventional cross-breeding of DAS-24236-5 and DAS-21Ø23-5, with insect resistance conferred by the cry1F gene from the common soil bacterium Bacillus thuringiensis var. aizawai and the cry1Ac gene from B. thuringiensis var. kurstaki.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)
    Crops
  • BCH-LMO-SCBD-14940-7 Living Modified Organism DAS-24236-5 - Insect-resistant cotton
    Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-LMO-SCBD-14938-7 Living Modified Organism DAS-21Ø23-5 - Insect-resistant cotton
    Dow AgroSciences | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
EN
Characteristics of the modification process
pMYC3006 and pAGM281
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
    Promoter
  • BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)
    Intron
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-103900-3 4ocs∆Mas2' promoter
    Promoter
DNA insert from 3006-210-23 vector pMYC3006:
Lepidopteran-resistant cotton, with insect resistance conferred by the cry1Ac gene from the common soil bacterium Bacillus thuringiensis (Bt) var. kurstaki strain HD73. Coding sequences of Cry1A(c) and pat genes altered for optimal expression in plant cells.

DNA insert from 281-24-236 vector pAGM281:
Lepidopteran-resistant cotton, with insect resistance conferred by the cry1F gene from the common soil bacterium Bacillus thuringiensis (Bt) var. aizawai. Coding sequences of Cry1F and pat genes altered for optimal expression in plant cells.

For additional information on this LMO, please refer to the records of the parental LMOs.
EN
LMO characteristics
EN
  • Fiber/textile
Additional Information
Cry proteins act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.
EN
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 10
Country's Decision or any other Communication Living modified organism(s) 11
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 3
Living Modified Organism Recipient Organism” or “Parental Organisms 1