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Modified Organism
SYN-E3272-5 - Enogen™ Maize
Record information and status
Record ID
15109
Status
Published
Date of creation
2006-06-19 11:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-05-08 19:58 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-05-08 19:58 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Enogen™ Maize
Transformation event
3272
Unique identifier
SYN-E3272-5
Developer(s)
Syngenta
Url:Syngenta
Description
Maize containing thermostable alpha-amylase (for optimised bioethanol production) through introduction of the amy797E gene from Thermococcales (thermostable bacterium). The pmi gene expresses the PMI protein, which allows the transformed plants to use mannose as an energy source and is used as a selectable marker.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
pNOV7013
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid right border repeat
0.03 Kb
 
 
27kD gamma-zein Promoter
0.68 Kb
 
 
amy797E alpha amylase
1.38 Kb
 
 
SEKDEL ER retention signal
0.01 Kb
 
 
Phosphoenolpyruvate carboxylase, intron 9
0.11 Kb
 
 
CaMV 35S terminator
0.07 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Ubiquitin Intron 1
0.00 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ti plasmid left border repeat
0.03 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Information on inserted DNA sequences
Corn Event 3272 contains single copies of the amy797E and pmi genes and single copies of the introduced GZein and ZmUbilnt promoters.

The chimeric amy797E gene was generated to combine the best features of three thermostable amylase enzymes and the coding region of the amy797E gene was modified for the preferred codon usage for maize.

The amy797E gene is fused to a 19 amino acid N-Terminal ER targeting sequence within the promoter sequence of GZein and the SEKDEL C-terminal ER retention signal.

Southern blot analysis indicated that single copies of each of the genetic elements was integrated into the host genome. Analysis indicated that there was no apparent integration of portions of the phosphate backbone. 
LMO characteristics
Modified traits
Common use(s)
  • Biofuel
Additional Information
Other relevant website address or attached documents

Records referencing this document (29)
IDDescription
29record(s) found
Country's Decision or any other Communication9 records
Modified Organism2 records
Organization8 records
Risk Assessment10 records