Enogen™ Maize

Maize containing thermostable alpha-amylase (for optimised bioethanol production) through introduction of the amy797E gene from Thermococcales (thermostable bacterium). The pmi gene expresses the PMI protein, which allows the transformed plants to use mannose as an energy source and is used as a selectable marker.

pNOV7013

Information on inserted DNA sequences
Corn Event 3272 contains single copies of the amy797E and pmi genes and single copies of the introduced GZein and ZmUbilnt promoters.

The chimeric amy797E gene was generated to combine the best features of three thermostable amylase enzymes and the coding region of the amy797E gene was modified for the preferred codon usage for maize.

The amy797E gene is fused to a 19 amino acid N-Terminal ER targeting sequence within the promoter sequence of GZein and the SEKDEL C-terminal ER retention signal.

Southern blot analysis indicated that single copies of each of the genetic elements was integrated into the host genome. Analysis indicated that there was no apparent integration of portions of the phosphate backbone.