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Modified Organism
DAS-24236-5 x DAS-21Ø23-5 x MON-88913-8 - Widestrike™ Roundup Ready Flex™ cotton
Record information and status
Record ID
15169
Status
Published
Date of creation
2006-06-20 10:51 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-02-12 21:37 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-02-12 21:37 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Widestrike™ Roundup Ready Flex™ cotton
Transformation event
281-24-236 x 3006-210-23 x MON88913
Unique identifier
DAS-24236-5 x DAS-21Ø23-5 x MON-88913-8
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
A stacked insect-resistant and glyphosate-tolerant cotton derived from conventional cross-breeding of DAS-21Ø23-5 x DAS-24236-5 with MON-88913-8.  Inserted genes include the cry1Ac gene from Bacillus thuringiensis subsp. kurstaki, the cry1F gene from Bacillus thuringiensis var. aizawai, the PAT encoding gene from Streptomyces viridochromogenes, and a naturally glyphosate tolerant form of the enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) from Agrobacterium tumefaciens strain CP4 to confer tolerance to phosphinothricin (glyphosate) herbicide.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
MON-88913-8 - Roundup Ready™ Flex™ cotton
Resistance to herbicides - Glyphosate
Show detection method(s)
DAS-21Ø23-5 - Insect-resistant cotton
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
DAS-24236-5 - Insect-resistant cotton
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
pAGM281, PV-GHGT35 and pMYC3006
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ubiquitin gene promoter
0.00 Kb
 
 
Ubiquitin Intron 1
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
ORF25 PolyA Terminator sequence
0.00 Kb
 
 
Cry1F
0.00 Kb
 
 
4ocs∆Mas2’ promoter
0.00 Kb
 
 
4ocs∆Mas2’ promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
ORF25 PolyA Terminator sequence
0.00 Kb
 
 
Cry1Ac
0.00 Kb
 
 
Ubiquitin gene promoter
0.00 Kb
 
 
FMV 35S Enhancer
0.00 Kb
 
 
Elongation factor EF-1alpha promoter
0.00 Kb
 
 
Elongation factor EF-1alpha Leader
0.00 Kb
 
 
Elongation factor EF-1alpha Intron 1
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
0.00 Kb
 
 
rbcS-E9 gene terminator
0.00 Kb
 
 
CaMV 35S Enhancer
0.00 Kb
 
 
Actin 8 Promoter
0.00 Kb
 
 
Actin 8 Leader sequence
0.00 Kb
 
 
Actin 8 Intron 1
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
0.00 Kb
 
 
rbcS-E9 gene terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from DAS-21Ø23-5, vector pMYC3006:
Lepidopteran-resistant cotton, with insect resistance conferred by the cry1Ac gene from the common soil bacterium Bacillus thuringiensis (Bt) var. kurstaki strain HD73. Also contains a coding sequence or the Phosphinothricin N-acetyltransferase gene which confers tolerance to glufosinate.

DNA insert from DAS-24236-5, vector pAGM281:
Lepidopteran-resistant cotton, with insect resistance conferred by the cry1F gene from the common soil bacterium Bacillus thuringiensis (Bt) var. aizawai. Also contains a coding sequence or the Phosphinothricin N-acetyltransferase gene which confers tolerance to glufosinate.

DNA insert from MON-88913-8, vector PV-GHGT35:
Roundup Ready® Flex cotton (MON 88913) was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option in cotton production. The transforming plasmid PV-GHGT35 carried a transfer DNA sequence comprising of two 5-enolpyruvylshikimate-3-phosphate synthase gene cassettes.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Fiber / Textile
Additional Information
Additional Information
DAS-21Ø23-5 x DAS-24236-5 x MON-88913-8 was produced by crossing WideStrike™ insect resistant cotton (DAS-21Ø23-5 x DAS-24236-5) with the herbicide-tolerant cotton line MON-88913-8. This stacked cotton line expresses four novel proteins: the delta-endotoxins Cry1F and Cry1Ac, which confer resistance to the lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm, the CP4 EPSPS protein, which confers tolerance to the herbicide glyphosate, and the PAT protein, which confers tolerance to glufosinate ammonium. The insecticidal proteins Cry1F and Cry1Ac are produced by the cry1F gene and cry1Ac genes respectively, both of which are from WideStrike™. The cotton line WideStrike™ was produced by crossing line 281-24-236 with line 3006-210-23. The PAT protein in WideStrike™ is produced by the pat gene: this expressed novel protein was intended solely for use as a selectable marker during plant transformation. The CP4 EPSPS protein is produced by the cp4 epsps gene from MON-88913-8.

This stacked cotton line is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.
Other relevant website address or attached documents

Records referencing this document (11)
IDDescription
11record(s) found
Country's Decision or any other Communication5 records
Information Resource1 record
Risk Assessment5 records