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Modified Organism
MON-88Ø17-3 x MON-ØØ81Ø-6 - YieldGard™ VT Triple
Record information and status
Record ID
15374
Status
Published
Date of creation
2006-07-12 16:21 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-07-24 14:43 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 14:43 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
YieldGard™ VT Triple
Transformation event
MON88017 x MON810
Unique identifier
MON-88Ø17-3 x MON-ØØ81Ø-6
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
A stacked insect-resistant maize derived from conventional cross-breeding of MON-88Ø17-3 and MON-ØØ81Ø-6.  Resistance to insect attack is conferred through expression of a truncated form of the cry1Ab gene and cry3Bb1 gene from Bacillus thuringiensis subsp. kurstaki HD-1 and tolerance to glyphosate-herbicides is produced through introduction of the epsps gene from Agrobacterium tumefaciens which confers tolerance to the herbicide Roundup, with the active ingredient glyphosate.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
MON-88Ø17-3 - YieldGard™ VT™ Rootworm/RR2™ Maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glyphosate
Show detection method(s)
MON-ØØ81Ø-6 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMIR39, PV-ZMBK07 and PV-ZMGT10
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Cry1Ab
3.46 Kb
 
 
Rice actin 1 gene promoter
0.93 Kb
 
 
Rice actin 1, intron
0.46 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.07 Kb
 
 
Rice actin 1, intron
0.46 Kb
 
 
Cry3Bb1
1.96 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.23 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON810, vectors PV-ZMBK07 and PV-ZMGT10
MON810 contains a truncated portion of a synthetic form of the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki. Two constructs PV-ZMBK07 and PV-ZMGT10 have been used for transformation, but molecular analyses showed that MON810 does not contain any element from PV-ZMGT10 construct and only the elements from construct PV-ZMBK07 have been integrated into its genome. MON810 contains one integrated DNA consisting of P-e35S, I-Hsp70 and cry1Ab. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.

DNA insert from MON88017 vector PV-ZMIR39
MON88017 expresses the cry3Bb1 gene encoding a Coleopteran-specific insecticidal protein to control infestation with corn root worm, and the cp4 epsps gene.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
MON88017 x MON810 is resistant to insect attack and tolerant to use of the herbicide glyphosate. The cry3Bb1 gene encodes a Coleopteran-specific insecticidal protein from Bacillus thuringiensis (subsp. kumamotoensis) in order to control infestation with corn root worm, the cry1Ab gene produces the insect control protein Cry1Ab, a delta-endotoxin which is insecticidal to lepidopteran insects, and the cp4 epsps gene from the soil bacterium Agrobacterium ssp. strain CP4 provides tolerance to the herbicide glyphosate.

The cry3Bb1 gene encodes the insect control protein Cry3Bb1, a delta-endotoxin, The toxin protects the plant from the Coleopteran insects western corn rootworm (Diabrotica virgifera), northern corn rootworm (D. barberi), and Mexican corn rootworm (D. virgifera zeae).  The cry1Ab gene produces the insect control protein Cry1Ab and protects the plant against lepidopteran insects such as the european corn borer (ECB).

Cry proteins act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. Cry3Bb1 is lethal only when eaten by Coleopteran species, including corn root worm, and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. Cry1Ab is insecticidal only when eaten by the larvae of lepidopteran insects (moths and butterflies). There are no binding sites for the delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.

The cp4 epsps gene encodes for a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that is highly tolerant to inhibition by glyphosate.

This line is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.
Other relevant website address or attached documents

Records referencing this document (27)
IDDescription
27record(s) found
Country's Decision or any other Communication9 records
Information Resource1 record
Organization7 records
Risk Assessment10 records