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Modified Organism
Canola MPS961 Phytaseed™ (phytase-producing )
Record information and status
Record ID
15379
Status
Published
Date of creation
2006-07-13 09:35 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-09-17 20:50 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-17 20:50 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Canola MPS961 Phytaseed™ (phytase-producing )
Transformation event
MPS961
Developer(s)
Description
Canola lines MPS961, MPS962, MPS963, MPS964 and MPS965 have been modified with the phyA gene to allow the plant to produce a fungal 3-phytase. This enzyme can be utilized to increase the breakdown of plant phytates which bind phosphorus. Phytate is the major storage form of phosphorus in many seeds and phytate-bound phosphorus is unavailable to monogastric animals. Since monogastric animals are not able to degrade this molecule, much of the phosphorus bound to phytate passes into the environment through the manure. Use of the enzyme and appropriate management techniques can lead to a reduction in the phosphorus content of manure, thus improving environmental conditions.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Point of collection or acquisition of the recipient organism
Variety: Westar
Related LMOs
Canola MPS962 Phytaseed™ (phytase-producing )
Changes in quality and/or metabolite content Phytate degradation Resistance to antibiotics - Kanamycin
Show detection method(s)
Canola MPS963 Phytaseed™ (phytase-producing)
Changes in quality and/or metabolite content Phytate degradation Resistance to antibiotics - Kanamycin
Show detection method(s)
Canola MPS964 Phytaseed™ (phytase-producing)
Changes in quality and/or metabolite content Phytate degradation Resistance to antibiotics - Kanamycin
Show detection method(s)
Canola MPS965 Phytaseed™ (phytase-producing)
Changes in quality and/or metabolite content Phytate degradation Resistance to antibiotics - Kanamycin
Show detection method(s)
Characteristics of the transformation process
Vector
pMOG625
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Cruciferin A gene promoter
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phyA gene
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Cruciferin A gene terminator
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The phytase gene was obtained from Aspergillus niger var. van Tieghem, and expression of this gene produces the enzyme 3-phytase. The nptII gene was isolated from transposon Tn5 in Klebsiella pneumonia. The nptII protein, neomycin phosphotransferase II, confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin, and was used by the firm as a selectable marker for transformed plant cells.

The novel genetic material in the new canola lines was inserted into the canola variety, Westar, using Agrobacterium tumefaciens-mediated transformation with the disarmed Ti-plasmid pMOG625. The T-DNA contained both phytase and nptII genes. The phytase gene is under the control of the cruciferin A seed storage protein transcript promoter which includes a cruciferin signal peptide sequence. Its terminator is also from the cruciferin A seed storage protein transcript. Both controlling sequences were obtained from Brassica napus. The nptII gene is under the control of the NOS promoter and terminator with an Agrobacterium tumefaciens-derived open reading frame inserted between the gene and its terminator. The open reading frame consists of coding for 50 amino acids from the Agrobacterium ornithine-cyclo-deaminase.
LMO characteristics
Modified traits
  • Changes in quality and/or metabolite content
Common use(s)
  • Food
  • Feed
Detection method(s)
Additional information
Lines MPS961, MPS962, MPS963 and MPS964 do not carry the bacterial selection marker nptII, nor the bacterial origin of replication as demonstrated by Southern analyses. Only MPS965 of the transgenic lines contains an expressed neomycin phosphotransferase II gene.

Records referencing this document (6)
IDDescription
6record(s) found
Country's Decision or any other Communication2 records
Modified Organism4 records